响应面法优化河蚬多肽的制备工艺  

Response Surface Methodology to Optimization of Prearation Process of Polypeptidefrom Corbicula fluminea

作　　者：刘依云 李思默 刘思宇 岳梅 毛璇 陈洪彬 LIU Yiyun;LI Simo;LIU Siyu;YUE Mei;MAO Xuan;CHEN Hongbin(College of Oceanology and Food Science,Quanzhou Normal University,Quanzhou 362000,China)

机构地区：[1]泉州师范学院海洋与食品学院,福建泉州362000

出　　处：《现代食品》2024年第7期81-86,共6页Modern Food

基　　金：泉州市科技计划项目(2020C066)。

摘　　要：为实现河蚬的高值化利用,本研究采用酶法制备河蚬多肽。以乙醇脱氢酶(Alcohol Dehydrogenase,ADH)激活率为指标,采用单因素和响应面法优化酶解工艺参数。结果表明,在料液比1∶130(g∶mL)、酶解时间140 min、酶解温度45℃的条件下,河蚬多肽对ADH的激活率为77.21%±0.82%。该研究为河蚬的精深加工及高值化开发利用提供了理论依据。In order to realize the high-value utilization of Corbicula fluminea,this study utilized an enzymatic method to prepare polypeptide from Corbicula fluminea.The alcohol dehydrogenase(ADH)activation rate were used as an indicators,and the enzymatic process parameters were optimized using one-factor and response surface.The results showed that under the conditions of solid to liquid ratio 1∶130(g∶mL),enzymolysis time 140 min and enzymolysis temperature 45℃,the activation rate of polypeptide on ADH was 77.21%±0.82%,the ADH activation rate of polypeptide from Corbicula fluminea was 77.21%±0.82%.This study provides a theoretical basis for the deep processing and high value utilization of Corbicula fluminea.

关 键 词：河蚬 酶解 工艺优化 ADH激活率 多肽 

分 类 号：S985.3[农业科学—捕捞与储运]