产细菌素海洋乳酸菌的筛选及细菌素抑菌机制  

Screening of Bacteriocin-producing Marine Lactic Acid Bacteria and Bacteriocin-inhibiting Mechanism

作　　者：赖崇熙 刘唤明 贾瑞博 邓楚津 洪鹏志 赵飞 LAI Chongxi;LIU Huanming;JIA Ruibo;DENG Chujin;HONG Pengzhi;ZHAO Fei(College of Food Science and Technology/Aquatic Product Processing Safety Key Lab of Guangdong Province/Guangdong Modern Agricultural Science and Technology Innovation Center/Guangdong Ocean University,Zhanjiang 524088,China;Guangdong Hengxing Feed Industry Co.,LTD,Zhanjiang 524022,China)

机构地区：[1]广东海洋大学食品科技学院/广东省水产品加工与安全重点实验室/广东省现代农业科技创新中心,广东湛江524088 [2]广东恒兴饲料实业股份有限公司,广东湛江524022

出　　处：《广东海洋大学学报》2024年第3期95-102,共8页Journal of Guangdong Ocean University

基　　金：湛江市科技计划(2020A02006)。

摘　　要：【目的】筛选海洋源产细菌素乳酸菌,探讨细菌素抑菌机制。【方法】从市售海鱼的肠道中筛选出一株对金黄色葡萄球菌(Staphylococcus aureus)和大肠埃希菌(Escherichia coli)有较强抑菌效果的菌株GOFEL226,通过16S rDNA序列鉴定乳酸菌,用乙酸乙酯提取细菌素,利用分光光度法、电导率法、扫描电镜技术探究细菌素的抑菌机制。【结果】菌株GOFEL226为戊糖乳植物杆菌(Lactiplantibacillus pentosus),GFEB226细菌素对金黄色葡萄球菌和大肠埃希菌的最小抑菌质量浓度为0.883 mg/mL;其对指示菌的生长有明显的抑制作用,可导致细胞的蛋白质、核苷酸、带电离子泄漏到细胞外,对细胞膜具有破坏作用。【结论】GFEB226细菌素可通过破坏革兰阳性和革兰阴性菌的细胞膜,使其胞内物质泄漏到胞外,进而导致细胞的死亡。【Objective】The aim of this paper is to screen bacteriocin-producing lactic acid bacteria from marine fish,and analyze the bacteriostatic mechanism of the bacteriocin.【Method】A strain of GOFEL226,which has strong antibacterial effect on Staphylococcus aureus and Escherichia coli,was screened from the intestinal tract of Marine fish.The species of lactobacillus was identified by 16S rDNA sequence analysis.Bacteriocin was extracted by ethyl acetate,and the antibacterial mechanism of bacteriocin was investigated by spectrophotometry,conductivity method and scanning electron microscopy.【Result】The strain GOFEL226 was Lactiplantibacillus pentosus,and the minimum inhibitory concentration(MIC)of GFEB226 bacteriocin against S.aureus and E.coli was 0.883 mg/mL.GFEB226 bacteriocin obviously inhibited the growth of indicator bacteria,leading to the leakage of proteins,nucleotides and charged ions in the cell to the outside of the cell,and damaging the cell membrane.【Conclusion】GFEB226 bacteriotin can damage the cell membrane of Gram-positive and Gram-negative bacteria,causing the leakage of intracellular substances to the outside of the cell,thus can cause cell death.

关 键 词：海洋乳酸菌 戊糖乳植物杆菌 细菌素 抑菌机理 抑菌活性 

分 类 号：R931.77[医药卫生—生药学]