拟微球藻不溶性膳食纤维提取工艺优化及其理化性质研究  

Optimization of extraction process of insoluble dietary fiber from Nannochloropsis gaditana and its physicochemical property study

作　　者：陈睿 张美兰 张蝶 张立伟 CHEN Rui;ZHANG Mei-lan;ZHANG Die;ZHANG Li-weil(College of Food Science and Engineering,Wuhan Polytechnic University,Wuhan 430023,Hubei,China;Key Laboratory of Deep Processing of Major Grain and Oil of Ministry of Education,Wuhan 430023,Hubei,China)

机构地区：[1]武汉轻工大学食品科学与工程学院,湖北武汉430023 [2]大宗粮油精深加工教育部重点实验室,湖北武汉430023

出　　处：《粮食与油脂》2024年第6期117-122,152,共7页Cereals & Oils

摘　　要：以拟微球藻藻粉为原料,采用超声辅助碱法提取不溶性膳食纤维(IDF),并对其理化特性进行研究。结果表明:最优提取条件为NaOH浓度1.8 mol/L、提取时间45 min、料液比1∶30(g/mL)、提取温度55℃,在此条件下藻粉IDF得率和纯度分别达到24.98%±0.93%和86.12%±0.25%。藻粉IDF理化指标分析显示,其持水力为(6.65±0.09)g/g、膨胀力为(6.25±0.18)mL/g、持油力为(1.90±0.03)g/g。体外吸附试验结果表明,藻粉IDF对NO_(2)^(-)、葡萄糖、胆固醇、胆酸钠均表现出较强的吸附能力。傅里叶红外光谱分析结果表明,藻粉IDF具有典型的多糖结构。Using Nannochloropsis gaditana powder as raw material,insoluble dietary fiber(IDF)was extracted by ultrasonic-assisted alkaline method,and its physicochemical property was investigated.The results indicated that the optimal extration conditions were NaOH concentration 1.8 mol/L,extraction time 45 min,solid-liquid ratio 1:30(g/mL)and extraction temperature 55℃.Under the conditions,the yield and purity of Nannochloropsis gaditanapowder IDF reached 24.98%±0.93%and 86.12%±0.25%,respectively.Analysis of the physicochemical indicators of Nannochloropsis gaditana powder IDF showed that its water-holding capacity was(6.65±0.09)g/g,swelling capacity was(6.25±0.18)mL/g,and oil-holding capacity was(1.90±0.03)g/g.The in vitro adsorption test results showed that the algal powder IDF exhibited strong adsorptioncapacity for NO_(2)^(-),glucose,cholesterol,and sodiumcholate.FT-IR infrared spectroscopy analysis indicated that Nannochloropsis gaditana powder IDF had typical polysaccharide structure.

关 键 词：拟微球藻 超声波辅助碱法 不溶性膳食纤维 理化性质 

分 类 号：TS201.1[轻工技术与工程—食品科学]