拟微绿球藻胞内多糖提取条件优化及其生物活性研究  

Optimization of Extraction Conditions and Bioactivity of Nannochloropsis Sp.Intracellular Polysaccharide

作　　者：刘松 刘红全 李慧敏 徐程浩 王燕姿 藏颖 LIU Song;LIU Hongquan;LI Huimin;XU Chenghao;WANG Yanzi;ZANG Ying(Guangxi Key Laboratory of Polysaccharide Materials and Modification,College of Marine Science and Biotechnology,Guangxi Minzu University,Nanning 530006,China)

机构地区：[1]广西民族大学海洋与生物技术学院,广西多糖材料与改性重点实验室,广西南宁530006

出　　处：《食品科技》2024年第3期233-242,共10页Food Science and Technology

基　　金：广西自然科学基金项目(2020487);广西自然科学基金项目(2023GXNSFAA026409)。

摘　　要：为研究拟微绿球藻胞内多糖(Nannochloropsis sp.intracellular polysaccharide,NOLP)提取工艺及其生物活性,通过单因素结合响应面法对拟微绿球藻胞内多糖提取条件进行优化,并对提取的多糖进行抗氧化和抑菌活性试验。结果表明,拟微绿球藻胞内多糖提取最佳条件为NaOH质量分数2.5%、料液比1:25 g/mL、超声功率100 W、超声时间10 min、水浴温度60℃,在此条件下,胞内多糖的提取率为(3.62±0.01)%。抗氧化试验结果显示,5 mg/mL拟微绿球藻胞内多糖对1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基的清除率为(78.57±0.85)%,对羟自由基的清除率为(22.48±0.65)%。体外抑菌试验结果表明,拟微绿球藻胞内多糖对蜡样芽孢杆菌、枯草芽孢杆菌、大肠杆菌有不同程度的抑制作用,选取抑制效果最强的蜡样芽孢杆菌为供试菌,测定多糖对菌体细胞膜结构的影响,试验结果表明,多糖对蜡样芽孢杆菌的细胞膜结构造成了一定程度的损伤,菌体的生长繁殖受到抑制。以上结果表明,NOLP具有良好的抗氧化活性及抑菌活性。In order to study the extraction technology and biological activity of Nannochloropsis sp.intracellular polysaccharide(NOLP),the extraction conditions of NOLP were optimized by single factor combined with response surface method,the antioxidant and bacteriostatic activities of the extracted NOLP were tested.The results showed that the optimal extraction parameters were NaOH mass fraction 2.5%,solid-liquid ratio 1:25 g/mL,ultrasonic power 100 W,ultrasonic time 10 min and water bath temperature 60℃.Under these conditions,the extraction rate of intracellular polysaccharide was(3.62±0.01)%.The antioxidant test results showed that the scavenging rate of 5 mg/mL intracellular polysaccharide to 1,1-diphenyl-2-picrylhydrazyl(DPPH)radical was(78.57±0.85)%,and the scavenging rate of hydroxyl free radical was(22.48±0.65)%.The results of antibacterial test in vitro showed that NOLP had different degrees of inhibitory effects on Bacillus cereus,Bacillus subtilis and Escherichia coli.The Bacillus cereus with the strongest inhibitory effect was selected as the test bacterium,and the influence of polysaccharide on the cell membrane structure of the bacteria was measured.The test results showed that polysaccharide caused damage to the cell membrane structure of Bacillus cereus to a certain extent.The growth and reproduction of thallus were inhibited.The above results showed that the NOLP has good antioxidant activity and antibacterial activity.

关 键 词：拟微绿球藻 多糖 提取条件优化 抗氧化活性 抑菌活性 

分 类 号：TS201.2[轻工技术与工程—食品科学]