马齿苋提取物对α-淀粉酶和α-葡萄糖苷酶的抑制作用  

Inhibitory Activities of Portulaca oleracea L.Extracts onα-Amylase andα-Glycosidase

作　　者：张义涛 王金兰[1,2] 张树军 赵英楠[1,2] 王丹 赵明 时志春[1,2] 李军 ZHANG Yitao;WANG Jinlan;ZHANG Shujun;ZHAO Yingnan;WANG Dan;ZHAO Ming;SHI Zhichun;LI Jun(Institute of Chemistry and Chemical Engineering,Qiqihar University,Qiqihar 161006,China;Heilongjiang Industrial Hemp Processing Technology Innovation Center,Technology Innovation Center of Industrial Hemp for State Market Regulation,Qiqihar 161006,China)

机构地区：[1]齐齐哈尔大学化学与化学工程学院,黑龙江齐齐哈尔161006 [2]黑龙江省工业大麻加工技术创新中心,国家市场监管技术创新中心(工业大麻),黑龙江齐齐哈尔161006

出　　处：《食品科技》2024年第3期243-249,共7页Food Science and Technology

基　　金：黑龙江省属高等学校基本科研业务费科研项目(145109512)。

摘　　要：目的:进一步研究干、鲜马齿苋的降血糖活性及降糖机制。方法:分别将干燥和新鲜马齿苋用乙醇为溶剂浸泡提取,制备干燥马齿苋乙醇提取物(Dried Portulaca oleracea L.ethanol extracts,DEP)和新鲜马齿苋乙醇提取物(Fresh Portulaca oleracea L.ethanol extracts,FEP)。再分别将DEP和FEP用水混悬,依次用正己烷、乙酸乙酯、正丁醇进行萃取,从DEP得到H-DEP、E-DEP、D-DEP和W-DEP,从FEP得到H-FEP、E-FEP、D-FEP和W-FEP。分别对DEP和FEP以及所得各种萃取物进行酶抑制活性测试,结果:E-FEP对2种酶的抑制作用最好,IC50值分别为(0.094±0.001)mg/mL和(33.63±0.42)μg/mL。绘制Lineweaver-Burk曲线确定酶抑制类型:对于α-淀粉酶,H-DEP、E-DEP、H-FEP及E-FEP为非竞争性抑制,DEP、FEP、D-DEP、W-DEP、D-FEP及W-FEP为竞争性和非竞争性混合抑制;对于α-葡萄糖苷酶,H-DEP、E-DEP、H-FEP及E-FEP为非竞争性抑制,DEP、FEP、D-DEP、W-DEP、D-FEP及W-FEP为反竞争性和非竞争性混合的抑制类型。结论:马齿苋具有良好的降血糖活性,该研究结果可为综合利用马齿苋资源提供理论依据,对研究开发新型降糖食物、推动降血糖活性研究进展等具有重要意义。Objective:To further investigate the hypoglycemic activity and hypoglycemic mechanism of dried and fresh Portulaca oleracea L.(purslane).Methods:Ethanol extracts were obtained from dried purslane(DEP)and fresh purslane(FEP),after filtration and concentrate.Ethanol extract of dried and fresh purslane were extracted with n-hexane,ethylacetate and n-butanol,the DEP was further separated by different polar solvents and obtained four components:H-DEP,E-DEP,D-DEP,and W-DEP.the FEP was further separated by different polar solvents and obtained four components:H-FEP,E-FEP,D-FEP,and W-DFP.The inhibition activity onα-amylase andα-glucosidase were determined.Results:E-FEP showed the best inhibition effect for both enzymes,with IC50 values of(0.094±0.001)mg/mL and(33.63±0.42)μg/mL.The Lineweaver-Burk curve was plotted to determine the type of enzyme inhibition.The results showed that H-DEP,E-DEP,H-FEP and E-FEP were sensitive toα-amylase,which were the type of noncompetitive inhibition.DEP,FEP,D-DEP,W-DEP,D-FEP and W-FEP were competitive and non-competitive mixed inhibition.Forα-glucosidases,the results showed that H-DEP,E-DEP,H-FEP and E-FEP were the type of noncompetitive inhibition.DEP,FEP,D-DEP,W-DEP,D-FEP and W-FEP were anti-competitive and non-competitive mixed inhibition type.Conclusion:Purslane has good hypoglycemic activity.The research results provide a theoretical basis for the comprehensive utilization of purslane resources,and are of great significance for the research and development of hypoglycemic foods and the advancement of hypoglycemic activity research.

关 键 词：马齿苋 Α-淀粉酶 Α-葡萄糖苷酶 抑制作用 酶动力学 

分 类 号：TS201.2[轻工技术与工程—食品科学]