粉丝中红薯、木薯成分荧光定量PCR检测方法研究  

Study on Fluorescent Quantitative PCR Detection Method of Sweet Potato and Cassava Components in Vermicelli

作　　者：陈晨 史国华 张瑞 张涛[1] 李永波[1] 贾文珅[2] 陈佳 周巍[1] CHEN Chen;SHI Guohua;ZHANG Rui;ZHANG Tao;LI Yongbo;JIA Wenshen;CHEN Jia;ZHOU Wei(Hebei Food Safety Key Laboratory,Key Laboratory of Special Food Supervision Technology for State Market Regulation,Hebei Engineering Research Center for Special Food Safety and Health,Hebei Food Inspection and Research Institute,Shijiazhuang 050227,China;Institute of Quality Standard and Testing Technology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100089,China;College of Chemical Technology,Shijiazhuang University,Shijiazhuang 050227,China)

机构地区：[1]河北省食品安全重点实验室,国家市场监管重点实验室(特殊食品监管技术),特殊食品安全与健康河北省工程研究中心,河北省食品检验研究院,河北石家庄050227 [2]北京市农林科学院,质量标准与检测技术研究所,北京100089 [3]石家庄学院化工学院,河北石家庄050227

出　　处：《食品科技》2024年第3期307-311,319,共6页Food Science and Technology

基　　金：河北省市场监督管理局科研计划项目(2022ZD11);河北省重点研发计划项目(21375501D);市场监督管理总局计划保障专项(2021YJ014)。

摘　　要：为实现粉丝中红薯、木薯源性成分的量化研究,实验应用荧光定量聚合酶链式反应(Polymerase chain reaction,PCR)对粉丝中红薯、木薯源性成分进行定性检测。针对目前市场上粉丝标签混乱的现状,分别在红薯及木薯的特异性基因上设计引物。该方法的建立能有效区分标签与实物不符的情况。根据荧光定量PCR检测结果显示,市场上标签显示为红薯的粉丝存在着木薯掺假现象。针对粉丝掺假现象目前还尚未建立标准,缺少该方面的监管。实验中建立的红薯木薯检测方法能对市场上存在的掺假现象起到一定的监督作用,为市场监管部门监管提供了科学依据,具有一定市场应用前景。In order to achieve quantitative research on sweet potato and cassava derived components in vermicelli,fluorescence quantitative polymerase chain reaction(PCR)was exployed to qualitatively detect sweet potato and cassava derived components in vermicelli.In response to the current chaotic situation of vermicelli labels in the market,primers were designed for specific genes in sweet potato and cassava.The establishment of this method can effectively identify situations where the label does not match the actual product.According to the results of fluorescence quantitative PCR detection,there is a phenomenon of cassava adulteration in the vermicelli labeled as sweet potato on the market.There are currently no standards established for vermicelli adulteration,and there is a lack of supervision in this area.The sweet potato and cassava detection method established in the experiment can play a certain supervisory role in the presence of adulteration in the market,provide scientific basis for market supervision departments,and have certain market application prospects.

关 键 词：荧光定量PCR 粉丝 植物源性成分 红薯 木薯 

分 类 号：TS207.3[轻工技术与工程—食品科学]