Neurotoxicity of tetramethylammonium ion on larval and juvenile zebrafish:Effects on neurobehaviors and multiple biomarkers  

作　　者：Ruixin Zhang Rui Wang Jiajun Chang GDaniel Sheng Daqiang Yin 

机构地区：[1]Key Laboratory of Yangtze River Water Environment,Ministry of Education,College of Environmental Science and Engineering,Tongji University,Shanghai 200092,China [2]Shanghai Institute of Pollution Control and Ecological Security,Shanghai 200092,China

出　　处：《Journal of Environmental Sciences》2024年第9期138-147,共10页环境科学学报（英文版）

基　　金：supported by the National Key Research and Development Program of China(No.2019YFC0408203)。

摘　　要：Tetramethylammonium hydroxide(TMAH)is an important compound that utilized and released by the rapidly expanding semiconductor industry,which could hardly be removed by the conventional wastewater treatment techniques.As a cholinergic agonist,the tetramethylammonium ion(TMA^(+))has been reported to induce toxicity to muscular and respiratory systems of mammals and human,however the toxicity on aquatic biota remains poorly known.We investigated the neurotoxic effects of TMA^(+)exposure on zebrafish,based on neurobehavior tests and a series of biomarkers.Significant inhibitions on the swimming distance of zebrafish larvae were observed when the exposure level exceeded 50 mg/L,and significant alterations on swimming path angles(straight and deflective movements)occurred even at 10 mg/L.The tested neurobehavioral endpoints of zebrafish larvae were significantly positively correlated with reactive oxygen species(ROS)and malondialdehyde(MDA),significantly negatively related with the activities of antioxidant enzymes,but not significantly correlated with the level of acetylcholinesterase(AChE).Such relationship indicates that the observed neurotoxic effects on swimming behavior of zebrafish larvae is mainly driven by oxidative stress,rather than the alterations of neurotransmitter.At the highest exposure concentration(200 mg/L),TMA^(+)evoked more severe toxicity on zebrafish juveniles,showing significantly stronger elevation on the MDA activity,and greater inhibitions on the activities of antioxidant enzymes and ACh E,suggesting juveniles were more susceptible to TMA^(+)exposure than larval zebrafish.

关 键 词：TETRAMETHYLAMMONIUM ZEBRAFISH Oxidative stress ACETYLCHOLINESTERASE NEUROBEHAVIOR 

分 类 号：X171.5[环境科学与工程—环境科学]