HS-SPME/GC-MS法比较不同改土方式对人参挥发性成分的影响  

Comparison of the Effects of Volatile Components of Panax ginseng by HS-SPME/GC-MS under Different Soil Modification Methods

作　　者：张庆贺 刘俊潼 田蓉 杨环旭 吴婷 李琼 陈长宝[1] 张涛[1] Zhang Qinghe;Liu Juntong;Tian Rong;Yang Huanxu;Wu Ting;Li Qiong;Chen Changbao;Zhang Tao*(Changchun University of Chinese Medicine,Changchun 130117,China)

机构地区：[1]长春中医药大学,吉林长春130117

出　　处：《中国野生植物资源》2024年第5期11-20,共10页Chinese Wild Plant Resources

基　　金：国家自然科学基金项目(82204556,82073969);吉林省科技发展计划项目(20220204083YY,YDZJ202303CGZH011);吉林省重点研发计划项目(20240305005YY)。

摘　　要：目的:分析比较5种不同土壤改良方式对老参地人参中挥发性成分的影响,为人参的栽培方式和进一步应用提供理论依据。方法:采用顶空-固相微萃取(HS-SPME)技术富集人参中挥发性成分,应用气相色谱-质谱联用技术(GC-MS)对检测出的成分进行分析与鉴定,采用面积归一化法确定各成分的相对含量。结果:人参样品中共鉴定出225个挥发性成分,其中烃类102种、酮类32种、醇酚类32种、醛类12种、酯类9种、杂环及其它类38种;老参地、强还原、强还原‑生物有机肥、氯化苦熏蒸、氯化苦熏蒸-强还原、氯化苦熏蒸-生物有机肥土壤条件下挥发性成分的质量分数分别为79.632%、96.104%、86.107%、96.512%、94.355%、90.476%,特有成分分别有20、23、16、19、27、32个,6种不同土壤条件下共有化合物8个。结论:不同土壤改良方式的人参中挥发性化学成分的含量和组成存在较大差异,为人参药材土壤改良方式的研究及挥发性成分的进一步应用提供借鉴。Objective:To compare the effects of five different soil modification methods and old ginseng soil on the changes of volatile components in the growth process of Panax ginseng and to provide a theoretical basis for cultivation methods of P.ginseng.Methods:The essential oils of P.ginseng were extracted by headspace solid phase microextraction(HS-SPME)and separated and identified by gas chromatography-mass spectrometry(GC-MS).The relative content of each component was determined through area normalization.Results:A total of 225 compounds were identified in the volatile constituents of P.ginseng from different soil modification methods,including 102 hydrocarbons,32 ketones,12 aldehydes,32 alcohol-phenols,9 esters,38 heterocycles and other compounds.The volatile components were identified under the cultivation conditions of old rhizome root,strong reduction,strong reduction-biological organic fertilizer,fumigation of bitter chloride,fumigation of bitter chloride-strong reduction and fumigation of bitter chloride-biological organic fertilizer accounted for 79.632%,96.104%,86.107%,96.512%,94.355%,90.476%,respectively.And there were 20,23,16,19,27 and 32 specific components of volatile oil in six ginseng samples,respectively,and 8 common components.Conclusion:There were significant differences in the content and composition of volatile chemical constituents in P.ginseng under six soil conditions,which provided reference for the study of soil improvement methods and further application of volatile components of P.ginseng.

关 键 词：人参 挥发性成分 改土方式 顶空-固相微萃取 气相色谱-质谱联用 

分 类 号：R284.1[医药卫生—中药学]