优质食品酶的催化机制及在高端配料创制方面的应用基础  

The Catalytic Mechanism of High-quality Food Enzymes and Their Application in the Creation of Upscale Ingredients

作　　者：李兆丰[1] 陈福生[2] 江正强[3] 王永华[4] 路福平 黄和[6] Li Zhaofeng;Chen Fusheng;Jiang Zhengqiang;Wang Yonghua;Lu Fuping;Huang He(School of Food Science and Technology,Jiangnan University,Wuxi 214122,Jiangsu;College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070;College of Food Science and Nutritional Engineering,China Agricultural University,Beijing 100083;School of Food Science and Engineering,South China University of Technology,Guangzhou 510641;National Engineering Laboratory for Industrial Enzymes,College of Biotechnology,Tianjin University of Science&Technology,Tianjin 300457;School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023)

机构地区：[1]江南大学食品学院,江苏无锡214122 [2]华中农业大学食品科学技术学院,武汉430070 [3]中国农业大学食品科学与营养工程学院,北京100083 [4]华南理工大学食品科学与工程学院,广州510641 [5]天津科技大学生物工程学院工业酶国家工程实验室,天津300457 [6]南京师范大学食品与制药工程学院,南京210023

出　　处：《中国食品学报》2024年第5期75-88,共14页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金面上项目(32272263);江苏省重点研发计划(现代农业)项目(BE2022323)。

摘　　要：优质食品酶是食品生物加工的核心,是食品制造产业的“芯片”。我国食品酶领域的应用基础研究薄弱,70%以上的酶制剂市场被国际公司垄断,优质食品酶长期受制于人,难以支撑目前食品行业提质升级的发展需求。在此背景下,本文聚焦新型食品酶高效筛选和分泌表达的生物学基础,高端高值食品酶的催化机制,食品酶催化性能改造的结构基础,食品酶创制高端配料的内在规律及作用机制等关键科学问题,综述优质食品酶的催化机制及在高端配料创制方面的应用基础,旨在为食品高端配料的酶法创制提供理论和实践指导。High-quality food enzymes are the core of food bioprocessing and the‘chip’of food manufacturing industry.However,the applied basic research of food enzymes in China is weak.More than 70%of the enzyme preparation market is monopolized by international companies.High-quality food enzymes have been restricted by others for a long time,which is difficult to support the development needs of the food industry.This review focused on the biological basis for efficient screening and secretion expression of novel food enzymes,the catalytic mechanism of high-quality and high-value food enzymes,the structural basis for improving the catalytic performance of food enzymes,the inherent law and mechanism of food enzymes to create upscale ingredients and other key scientific issues.The catalytic mechanism of high-quality food enzymes and their application in the creation of upscale ingredients was summarized.This review aimed to provide theoretical and practical guidance for the enzymatic production of upscale food ingredients.

关 键 词：新型食品酶 催化机制 结构基础 高端配料 

分 类 号：TS201.25[轻工技术与工程—食品科学]