跨越式滚环等温扩增试剂盒检测沙门氏菌的研究  

Study on the Detection of Salmonella Using a Detection Kit Based on Saltatory Rolling Circle Amplification

作　　者：杨倩 张欣[2] 张金库[3] 耿凤珍[4] 王明玉 李艳玲 YANG Qian;ZHANG Xin;ZHANG Jinku;GENG Fengzhen;WANG Mingyu;LI Yanling(School of Public Health,Hebei University,Baoding 071000,China;Public Health Department of Baoding First Central Hospital,Baoding 071000,China;Key Laboratory of Molecular Pathology and Early Diagnosis of Tumors in Hebei Province,Department of Pathology,Baoding First Central Hospital,Baoding 071000,China;Laboratory Department of Hebei University Affiliated Hospital,Baoding 071000,China;Pharmacy Department of Hebei University Affiliated Hospital,Baoding 071000,China;Baoding Disease Prevention and Control Center,Baoding 071000,China)

机构地区：[1]河北大学公共卫生学院,河北保定071000 [2]保定市第一中心医院公共卫生处,河北保定071000 [3]河北省分子病理与肿瘤早期诊断重点实验室,保定市第一中心医院病理科,河北保定071000 [4]河北大学附属医院检验科,河北保定071000 [5]河北大学附属医院药学部,河北保定071000 [6]保定市疾病预防控制中心,河北保定071000

出　　处：《食品安全导刊》2024年第5期109-112,共4页China Food Safety Magazine

基　　金：河北省高层次人才资助项目(C20231100);河北省高等学校科学技术研究项目(QN2022073);河北大学校长基金(XZJJ202314);河北大学医学学科培育项目重点项目(2022A06);河北大学高层次人才科研启动项目(521100222024);河北大学大学生创新训练计划项目(DC2024235)。

摘　　要：沙门氏菌可引起食源性疾病,严重危害食品安全和人类健康。因此,开发新的检测方法尤为重要。本研究基于自主研发的新型核酸扩增方法--跨越式滚环等温扩增,研制了检测沙门氏菌的试剂盒,并对该试剂盒相关性能进行评估。结果显示,试剂盒检测沙门氏菌的特异性良好,灵敏度为4.1×10^(1)CFU·mL^(-1),有效期可达90 d,表明该试剂盒可靠、灵敏、稳定,可满足食品中沙门氏菌快速检测的需求。Salmonella can cause foodborne diseases and seriously endanger food safety and human health.Therefore,the development of new detection methods is particularly important.This study is focused on the application of a novel nucleic acid amplification method,saltatory rolling circle amplification,developed independently by our research team.We utilized this method to create a detection kit specifically designed for Salmonella,and the performance of this kit was evaluated.The results demonstrate that the kit exhibits excellent specificity for detecting Salmonella,with a sensitivity of 4.1×10^(1) CFU·mL^(-1) and a shelf life of up to 90 days.These results indicate that the detection kit is reliable,sensitive,and stable,thus meeting the demand for rapid Salmonella testing in food products.

关 键 词：沙门氏菌 跨越式滚环等温扩增 检测试剂盒 

分 类 号：TS201.3[轻工技术与工程—食品科学]