Hypoxia-preconditioned bone marrow-derived mesenchymal stem cells protect neurons from cardiac arrest-induced pyroptosis  

作　　者：Xiahong Tang Nan Zheng Qingming Lin Yan You Zheng Gong Yangping Zhuang Jiali Wu Yu Wang Hanlin Huang Jun Ke Feng Chen 

机构地区：[1]Shengli Clinical Medical College of Fujian Medical University,Fujian Medical University,Fuzhou,Fujian Province,China [2]Department of Emergency,Fujian Provincial Hospital,Fuzhou,Fujian Province,China [3]Fujian Provincial Key Laboratory of Emergency Medicine,Fuzhou,Fujian Province,China [4]The Second Department of Intensive Care Unit,Fujian Provincial Hospital South Branch,Fuzhou,Fujian Province,China

出　　处：《Neural Regeneration Research》2025年第4期1103-1123,共21页中国神经再生研究（英文版）

基　　金：supported by the Natural Science Fund of Fujian Province,No.2020J011058(to JK);the Project of Fujian Provincial Hospital for High-level Hospital Construction,No.2020HSJJ12(to JK);the Fujian Provincial Finance Department Special Fund,No.(2021)848(to FC);the Fujian Provincial Major Scientific and Technological Special Projects on Health,No.2022ZD01008(to FC).

摘　　要：Cardiac arrest can lead to severe neurological impairment as a result of inflammation,mitochondrial dysfunction,and post-cardiopulmonary resuscitation neurological damage.Hypoxic preconditioning has been shown to improve migration and survival of bone marrow–derived mesenchymal stem cells and reduce pyroptosis after cardiac arrest,but the specific mechanisms by which hypoxia-preconditioned bone marrow–derived mesenchymal stem cells protect against brain injury after cardiac arrest are unknown.To this end,we established an in vitro co-culture model of bone marrow–derived mesenchymal stem cells and oxygen–glucose deprived primary neurons and found that hypoxic preconditioning enhanced the protective effect of bone marrow stromal stem cells against neuronal pyroptosis,possibly through inhibition of the MAPK and nuclear factor κB pathways.Subsequently,we transplanted hypoxia-preconditioned bone marrow–derived mesenchymal stem cells into the lateral ventricle after the return of spontaneous circulation in an 8-minute cardiac arrest rat model induced by asphyxia.The results showed that hypoxia-preconditioned bone marrow–derived mesenchymal stem cells significantly reduced cardiac arrest–induced neuronal pyroptosis,oxidative stress,and mitochondrial damage,whereas knockdown of the liver isoform of phosphofructokinase in bone marrow–derived mesenchymal stem cells inhibited these effects.To conclude,hypoxia-preconditioned bone marrow–derived mesenchymal stem cells offer a promising therapeutic approach for neuronal injury following cardiac arrest,and their beneficial effects are potentially associated with increased expression of the liver isoform of phosphofructokinase following hypoxic preconditioning.

关 键 词：bone marrow–derived mesenchymal stem cells cardiac arrest cardiac resuscitation hypoxic preconditioning liver isoform of phosphofructokinase mitochondria NEUROINFLAMMATION oxidative stress PYROPTOSIS reactive oxygen species 

分 类 号：R741[医药卫生—神经病学与精神病学]