A non-viral gene therapy for melanoma by staphylococcal enterotoxin A  

作　　者：Ling Yang Min Ren Jie Wang Liming He Shanshan Wu Shuai Yang Wei Zhao Hao Cheng Xiaoming Zhou Maling Gou 

机构地区：[1]State Key Laboratory of Biotherapy and Cancer Center,West China Hospital,Sichuan University,Chengdu 610041,China [2]Department of Ophthalmology,West China Hospital,Sichuan University,Chengdu 610041,China [3]State Key Laboratory of Biotherapy,Department of Integrated Traditional Chinese and Western Medicine,Rare Diseases Center,West China Hospital,Sichuan University,Chengdu 610041,China

出　　处：《Chinese Chemical Letters》2024年第5期325-329,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation(No.82073363);the Sichuan Science and Technology Program(Nos.2020YFQ0059,2022YFQ0004);the Natural Science Foundation of Sichuan Province(No.2022NSFSC1304).

摘　　要：Staphylococcal enterotoxin A(SEA)derived from Staphylococcus aureus,as a superantigen,shows potential for cancer immunotherapy,but systemic immunotoxicity restricts its clinical application.Targeted delivery of SEA to tumor site provides a promising option for reducing the systemic toxicity.Here,we constructed an iRGD peptide(H-[Cys-Arg-Gly-Asp-Lys-Gly-Pro-Asp-Cys]-NH_(2))modified nanoparticle(iDPP)to deliver plasmids encoding SEA for melanoma treatment.The iDPP/SEA nanocomplexes efficiently mediated SEA expression in B16-F10 cells in vivo and in vitro and induced the activation of lymphocytes and maturation of murine bone marrow-derived dendritic cells(BMDCs)in vitro.In the subcutaneous B16-F10 melanoma model,the iDPP/SEA nanocomplexes could effectively enhance immune response and T lymphocytes infiltration in tumor site after intravenous administration,thereby considerably decreased melanoma growth.Meanwhile,no obvious adverse effect was observed after intravenous administration of the iDPP/SEA nanocomplexes in vivo.Our findings demonstrated that gene therapy of SEA is a potential candidate for melanoma treatment.

关 键 词：Gene therapy SUPERANTIGEN MELANOMA Staphylococcal enterotoxins A Immunotherapyene therapy 

分 类 号：R739.5[医药卫生—肿瘤]