苦参多糖的提取工艺优化及体外抗氧化、降血脂活性分析  

Optimization of the Extraction Process and in Vitro Antioxidant and Hypolipidemic Activities of Polysaccharides from Sophora flavescens

作　　者：赵小亮 牟翮 张晶 张伟杰[1,2] ZHAO Xiaoliang;MOU He;ZHANG Jing;ZHANG Weijie(College of Life Science and Engineering,Lanzhou University of Science and Technology,Lanzhou 730050,China;Key Laboratory of Screening,Evaluation and Deep Processing of Chinese and Tibetan Medicines in Gansu Province,Lanzhou 730050,China)

机构地区：[1]兰州理工大学生命科学与工程学院,甘肃兰州730050 [2]甘肃省中藏药筛选评价及深加工高校重点实验室,甘肃兰州730050

出　　处：《食品工业科技》2024年第13期212-220,共9页Science and Technology of Food Industry

基　　金：国家自然科学基金项目(32260231,31860250);甘肃省高校教师创新基金项目(2023A-020);兰州市青年科技人才创新项目(2023-QN-65)。

摘　　要：目的:为提高苦参多糖的得率,明确多糖的抗氧化和降血脂活性,通过正交试验优化多糖的提取工艺,并评价其体外抗氧化和降血脂活性。方法:以多糖得率为评价指标,在单因素实验研究提取温度、提取次数、料液比和提取时间对苦参多糖得率影响的基础上,通过正交试验优化多糖的提取工艺。通过对DPPH自由基、羟基自由基和超氧阴离子自由基的清除作用评价多糖的体外抗氧化活性,通过与胆酸钠(SC)、牛磺胆酸钠(STC)、甘氨胆酸钠(SGC)和混合胆酸盐(MBS)的结合作用评价多糖的体外降血脂活性。结果:苦参多糖的最佳提取条件为:温度85℃,料液比1:35 (g:mL),提取时间3.5 h,在此条件下多糖平均得率为3.07%±0.07%。苦参多糖在浓度为5 mg/mL时对DPPH自由基、羟基自由基和超氧阴离子自由基的清除率分别为60.1%±3.6%、39.6%±3.1%和45.1%±4.1%;在浓度为10 mg/mL时与胆酸钠(SC)、牛磺胆酸钠(STC)、甘氨胆酸钠(SGC)和混合胆酸盐(MBS)的结合率分别为34.4%±0.75%、45.4%±0.15%、19.7%±0.94%和37.7%±0.64%。结论:苦参多糖具有较好的体外抗氧化和降血脂活性。本研究为苦参多糖的开发利用提供了一定的理论依据。Objective:To increase the yield of Sophora flavescens polysaccharides and elucidate their antioxidant and hypolipidemic properties,this study optimised the polysaccharide extraction process through orthogonal experiments,followed by evaluation of their antioxidant and hypolipidemic activities in vitro.Methods:Using polysaccharide yield as the primary metric,this study performed orthogonal experiments to optimise the extraction process,building on preliminary one-way experiments.This study investigated the effect of extraction temperature,number of extractions,material-liquid ratio and extraction time on the polysaccharide yield of Sophora flavescens.Furthermore,this study evaluated the in vitro antioxidant activity of the polysaccharides by investigating their scavenging potential against DPPH radicals,hydroxyl radicals and superoxide anion radicals.In addition,the in vitro hypolipidemic activity of the polysaccharides was evaluated by their binding affinity to sodium cholate(SC),sodium taurocholate(STC),sodium glycylcholate(SGC)and mixed bile salts (MBS). Results: The optimal conditions for the extraction of Sophora flavescens polysaccharide were determined as follows: a temperature of 85 ℃, a material-liquid ratio of 1:35 (g:mL), and an extraction duration of 3.5 hours, yielding an average polysaccharide content of 3.07%±0.07%. The scavenging efficiencies of Sophora flavescens polysaccharides against DPPH radicals, hydroxyl radicals, and superoxide anion radicals at a concentration of 5 mg/mL were measured at 60.1%±3.6%, 39.6%±3.1% and 45.1%±4.1%, respectively. Furthermore, the binding affinities of Sophora flavescens polysaccharides to SC, STC, SGC and MBS at a concentration of 10 mg/mL were determined to be 34.4%±0.75%, 45.4%±0.15%, 19.7%±0.94% and 37.7%±0.64%, respectively. Conclusion: Sophora flavescens polysaccharide exhibits notable in vitro antioxidant and hypolipidemic activities. This investigation furnishes a foundational understanding for the potential development and exploitation of Sophor

关 键 词：苦参 多糖 工艺优化 抗氧化活性 降血脂活性 

分 类 号：TS255.1[轻工技术与工程—农产品加工及贮藏工程]