1株甜菜糖蜜源高产胞外多糖明串珠菌的分离鉴定、发酵工艺优化及抗氧化活性研究  

Isolation and identification,fermentation conditions process optimization and antioxidant activity of a high exopolysaccharides-producing leuconostoc sp.from sugar beet molasses

作　　者：张菡 陈海军 龙晓宇 赵抒娜 魏学军 魏国坤 申光辉[2] ZHANG Han;CHEN Haijun;LONG Xiaoyu;ZHAO Shuna;WEI Xuejun;WEI Guokun;SHEN Guanghui(National Postdoctoral Programme/Xinjiang Engineering Research Center of Beet Sugar/Key Laboratory of Quality Sugar and Safety Control for Sugar Crops and Tomato Process Technology Innovation,Ministry of Agriculture and Rural Affairs,COFCO Sugar Holding Co.,Ltd.,Changji 831100,China;College of Food Science/Key Laboratory of Agro-Products Processing and Nutritional Health(Co-Construction by Ministry and Province),Ministry of Agriculture and Rural Affairs,Sichuan Agricultural University,Ya′an 625014,China;School of Food and Health,Beijing Technology and Business University,Beijing 100048,China)

机构地区：[1]中粮糖业控股股份有限公司国家博士后科研工作站/新疆甜菜制糖工程研究中心/农业农村部糖与番茄加工技术创新重点实验室,新疆昌吉831100 [2]四川农业大学食品学院/农业农村部农产品加工与营养健康重点实验室(部省共建),四川雅安625014 [3]北京工商大学食品与健康学院,北京100048

出　　处：《轻工学报》2024年第3期9-20,共12页Journal of Light Industry

基　　金：2022年新疆维吾尔自治区专利转化项目(20220023);昌吉回族自治州科技计划项目(2022X01);中粮糖业控股股份有限公司-四川农业大学校企合作项目(060-2222319002);2021年度新疆维吾尔自治区昌吉回族自治州博峰精英计划柔性领军人才项目(2022015)。

摘　　要：为挖掘甜菜糖蜜中高产胞外多糖乳酸菌菌种资源,对分离自甜菜糖蜜中的1株产胞外多糖菌株M1进行分类鉴定,分析其生长特性;采用单因素试验结合Box-Behnken响应面法优化该菌株利用甜菜糖蜜作为底物发酵胞外多糖的发酵工艺参数,并对提取的粗胞外多糖的抗氧化活性进行分析。结果表明:菌株M1为明串珠菌属的苏奥古姆明串珠菌(Leuconostoc suionicum),其延滞期短(仅2 h),最适生长温度为35℃,最适pH值为8.0,最高可耐受质量分数为60%的蔗糖;优化后的胞外多糖发酵培养基为甜菜糖蜜300.0 g/L,酵母粉20.0 g/L,K 2HPO_(4)·3H_(2)O 15.0 g/L,MgSO_(4)·7H_(2)O 0.2 g/L,NaCl 0.01 g/L,CaCl_(2)·2H_(2)O 0.01 g/L,MnSO_(4)·4H_(2)O 0.01 g/L,FeSO_(4)·7H_(2)O 0.01 g/L;最优发酵工艺参数为装液量200 mL/250 mL、接种量5%、初始pH值8.1、发酵温度38.5℃、发酵时间41.1 h,此条件下的胞外多糖产量最高,为39.1 g/L;菌株M1所产胞外多糖具有良好的抗氧化活性,质量浓度为6.0 mg/mL的胞外多糖对DPPH自由基、·OH自由基和ABTS+自由基的清除率分别为70.25%、47.16%和35.92%,对Fe^(3+)的总还原力为0.42。菌株M1能够利用甜菜糖蜜发酵生产具有较好抗氧化活性的胞外多糖,在生物转化甜菜糖蜜生产功能性胞外多糖产品方面具有良好的应用前景。The objective of this study was to screen lactic acid bacteria strains with high exopolysaccharides production potential from sugar beet molasses and to evaluate their exopolysaccharides production capacity using molasses as carbon source.The taxonomic identification and growth characteristics of a lactic acid bacteria strain M1 with high exopolysaccharides-producing capacity isolated from sugar beet molasses were investigated.Furthermore,the exopolysaccharides fermentation parameters using sugar beet molasses as an alternative carbon source to sucrose were optimized using a single-factor experiment and response surface methodology based on Box-Behnken design.Moreover,antioxidant activity of the harvested crude exopolysaccharides were evaluated.The results showed that strain M1 was taxonomically classified as Leuconostoc suionicum.The growth profile of L.suionicum M1 indicated a latency period lag phase of 2 hours,an optimal temperature of 35℃,a favorable pH value of 8.0,and a remarkable tolerance to sucrose concentrations up to 60%.The optimal medium for exopolysaccharides production consisted of 300 g/L sugar beet molasses,20.0 g/L yeast extract,15.0 g/L K 2HPO 4·3H_(2)O,0.2 g/L MgSO 4·7H_(2)O,0.01 g/L NaCl,0.01 g/L CaCl_(2)·2H_(2)O,0.01 g/L MnSO 4·4H_(2)O,0.01 g/L FeSO 4·7H_(2)O.The maximum exopolysaccharides yield of 39.1 g/L was achieved under the following conditions:loading volume 200 mL/250 mL,inoculum size 5%,initial pH value 8.1,fermentation temperature of 38.5℃and fermentation period 41.1 h.The in vitro result of antioxidant activity showed that the harvested exopolysaccharides produced by strain M1 exhibited significant antioxidant activities,with quenching capacities of 70.25%for DPPH radicals,47.16%for hydroxyl·OH radicals,35.92%for ABTS+radicals and 0.42 for Fe^(3+)total reducing force at a concentration of 6.0 mg/mL.L.suionicum Strain M1 was capable of producing exopolysaccharides with antioxidant activity using sugar beet molasses,and could be used as a promising candidate for the bioco

关 键 词：苏奥古姆明串珠菌 甜菜糖蜜 胞外多糖 发酵工艺 抗氧化活性 

分 类 号：TS201.3[轻工技术与工程—食品科学] TS249.3[轻工技术与工程—食品科学与工程]