响应面法优化乌龙茶提取茶多糖的工艺研究  

Optimization of Extraction Process of Tea Polysaccharide from OolongTea Based on Response Surface Methodology

作　　者：刘舜慧[1,4] 吕淑珍 李少华 王二丽[1] 张怡评[4] LIU Shunhui;LYU Shuzhen;LI Shaohua;WANG Erli;ZHANG Yiping(College of Pharmacy,Zhangzhou Health Vocational College,Zhangzhou Fujian 363000,China;School of Marine Biology,Xiamen Ocean Vocational College,Xiamen Fujian 351005,China;Department of Rehabilitation,Wuyi University,Wuyishan Fujian 354300,China;Technology Innovation Center for Exploitation of Marine Biological Resources,Third Institute of Oceannography Ministry of Natural Resources,Xiamen Fujian 351005,China)

机构地区：[1]漳州卫生职业学院药学院,福建漳州363000 [2]厦门海洋职业技术学院海洋生物学院,福建厦门351005 [3]武夷学院康复治疗系,福建武夷山354300 [4]自然资源部第三海洋研究所海洋生物资源开发利用工程技术创新中心,福建厦门351005

出　　处：《鞍山师范学院学报》2024年第2期43-49,共7页Journal of Anshan Normal University

基　　金：福建省科技计划项目(2021N0029);厦门市科技计划项目(3502Z20221036);漳州卫生职业学院2022年访问学者项目(漳卫职人〔2022〕1号).

摘　　要：以乌龙茶为原料,选用回流法提取茶多糖,根据Plackett-Burman实验设计考察提取温度、提取时间、提取次数、料液比等多种因素对茶多糖提取率的影响;在单因素实验基础上,运用Box-Behnken实验设计进行响应面剖析.实验结果显示,当提取温度100℃、提取时长80 min、料液比1∶50时,乌龙茶茶多糖的提取率显著增加,得率是6.13%.对最佳提取工艺开展验证,结果显示实测均值与模型估计值相近,表明该提取工艺平稳可行.With oolong tea as the raw material,the tea polysaccharide was extracted by reflux method.According to Plackett-Burman experiment,the effects of extraction temperature,extraction duration,extraction frequency and solid-liquid check on the extraction rate of tea polysaccharide were studied.Under the premise of single factor experiment,the box Behnken experiment is designed and the response surface is analyzed.The results showed that when the extraction temperature was 100℃,the extraction period was 80 min,and the ratio of solid to liquid was 1∶50,the extraction rate of oolong tea polysaccharide was significantly increased,and the yield was 6.13%.The best extraction processing technology is proved,and the evaluation mean value is close to the estimated value of the entity model,indicating that the extraction processing technology is stable and feasible.

关 键 词：乌龙茶 茶多糖 单因素实验 响应面法 PLACKETT-BURMAN Box-Behnken 

分 类 号：TS272[农业科学—茶叶生产加工]