红茶的体外模拟消化及TFDG对胰脂肪酶活性的抑制作用  

In vitro Simulated Digestion of Black Tea and Inhibitory Effect of TFDG on Pancreatic Lipase Activity

作　　者：陈光萱 袁慧聪 王旭[1] CHEN Guangxuan;YUAN Huicong;WANG Xu(School of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China)

机构地区：[1]天津科技大学食品科学与工程学院,天津300457

出　　处：《食品研究与开发》2024年第12期59-65,共7页Food Research and Development

摘　　要：肥胖已成为影响人们生活方式的现代流行病,抑制胰脂肪酶的活性是治疗肥胖的关键。该研究考察红茶经体外模拟消化后酚类物质的生物可及性和抗肥胖特性,并通过荧光光谱探究茶黄素-3,3′-双没食子酸酯(theaflavin-3,3′-digallate,TFDG)对胰脂肪酶活性的抑制机制。体外模拟消化试验结果表明,红茶在消化过程中酚类物质含量降低,最终生物可及性维持在32.04%,但经体外消化后其对胰脂肪酶活性的抑制率增加26.97%,并且有效地抑制了膳食脂肪的分解。荧光光谱分析结果表明TFDG能够静态猝灭胰脂肪酶的内源荧光,氢键和疏水作用是二者结合过程中的主要驱动力,并且在结合过程中胰脂肪酶的构象发生改变。Obesity has become a modern epidemic affecting human lifestyles around the world.Inhibiting the activity of pancreatic lipase is the key to the treatment of obesity.In this study,the bioaccessibility and anti⁃obesity properties of polyphenols from black tea after in vitro simulated digestion were investigated,and the in⁃hibitory mechanism of theaflavin⁃3,3'⁃digallate(TFDG)on pancreatic lipase activity was explored by fluores⁃cence spectroscopy.The results of in vitro simulated digestion experiments showed that the phenolic content of black tea decreased,and the final bioaccessibility was maintained at 32.04%.However,after in vitro digestion,the inhibition rate of black tea on pancreatic lipase increased by 26.97%,which effectively inhibited the hydro⁃lysis of dietary lipid.The results of fluorescence analysis showed that TFDG could statically quench the intrin⁃sic fluorescence of pancreatic lipase.Hydrogen bonding and hydrophobic interaction were the main driving forces in the binding process of the two,and the conformation of pancreatic lipase had changed in the binding process.

关 键 词：胰脂肪酶 红茶 茶黄素-3 3′-双没食子酸酯 体外模拟消化 荧光光谱 

分 类 号：TS272.52[农业科学—茶叶生产加工]