CRISPR-Cas系统在食源性致病菌检测中的研究进展  

Research progress of CRISPR-Cas system in the detection of foodborne pathogens

作　　者：郭浩宇 王瑞曦 秦琳琳 王邵天 于淏芃 赵枳熒 尹丹阳 马龙 GUO Hao-Yu;WANG Rui-Xi;QIN Lin-Lin;WANG Shao-Tian;YU Hao-Peng;ZHAO Zhi-Ying;YIN Dan-Yang;MA Long(State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science and Technology College of Biotechnology,Tianjin 300457,China;Tianjin University of Science and Technology College of Art and Design,Tianjin 300457,China)

机构地区：[1]天津科技大学生物工程学院,食品营养与安全国家重点实验室,天津300457 [2]天津科技大学艺术设计学院,天津300457

出　　处：《食品安全质量检测学报》2024年第8期154-163,共10页Journal of Food Safety and Quality

基　　金：国家自然科学基金项目(32072309,32372415);天津市科技计划项目(19JCYBJC27800,22YFZCSN00070,23JCJQJC00090);国家级大学生创新创业训练计划资助项目(202310057123);校级大学生创新创业训练计划资助项目(202310057136)。

摘　　要：食源性致病菌的快速检测对预防食源性疾病的爆发具有重要意义。成簇规律间隔短回文重复序列(clustered regularly interspaced short palindromicrepeats,CRISPR)及CRISPR相关蛋白(CRISPR-associated protein,Cas)构成的CRISPR-Cas系统已被广泛的应用于核酸检测,并在对食源性致病菌的检测中展现出快速、灵敏度高、特异性强等优势。本文介绍了CRISPR-Cas系统的原理、机制,总结了CRISPR-Cas系统与不同扩增方法和信号输出模式相结合在食源性致病菌检测中的应用,并讨论了这些检测方法存在的一些问题和挑战,最后对该技术的未来发展前景进行展望,旨在为食源性致病菌的快速检测提供新思路。Rapid detection of foodborne pathogens is of great significance in preventing the outbreak of foodborne diseases.Clustered regularly interspaced short palindromic repeats(CRISPR)and CRISPR-associated protein,the CRISPR/Cas system composed has been widely used in nucleic acid detection,and has shown advantages such as fast,high sensitivity and strong specificity in the detection of foodborne pathogens.This paper introduced the principle and mechanism of CRISPR/Cas system,summarized the application of CRISPR/Cas system combined with different amplification methods and signal output modes in the detection of foodborne pathogens,and discussed some problems and challenges existing in these detection methods.Finally,the future development prospect of this technology was prospected.The aim is to provide a new idea for the rapid detection of foodborne pathogens.

关 键 词：成簇规律间隔短回文重复序列相关蛋白 食源性致病菌 核酸检测 

分 类 号：TS207.4[轻工技术与工程—食品科学]