Multimodal Nature of the Single-cell Primate Brain Atlas:Morphology,Transcriptome,Electrophysiology,and Connectivity  

作　　者：Yuhui Shen Mingting Shao Zhao-Zhe Hao Mengyao Huang Nana Xu Sheng Liu 

机构地区：[1]State Key Laboratory of Ophthalmology,Zhongshan Ophthalmic Center,Sun Yat-sen University,Guangdong Provincial Key Laboratory of Ophthalmology and Visual Science,Guangzhou,510060,China [2]Guangdong Province Key Laboratory of Brain Function and Disease,Guangzhou,510080,China

出　　处：《Neuroscience Bulletin》2024年第4期517-532,共16页神经科学通报（英文版）

基　　金：supported by the Natural Science Foundation of China(81961128021 and 82371095);the National Key R&D Program of China(2022YEF0203200);the Guangdong Provincial Key R&D Programs(2018B030335001);the Science and Technology Program of Guangzhou(202007030011 and 202007030010).

摘　　要：Primates exhibit complex brain structures that augment cognitive function.The neocortex fulfills high-cognitive functions through billions of connected neurons.These neurons have distinct transcriptomic,morphological,and electrophysiological properties,and their connectivity principles vary.These features endow the primate brain atlas with a multimodal nature.The recent integration of next-generation sequencing with modified patch-clamp techniques is revolutionizing the way to census the primate neocortex,enabling a multimodal neuronal atlas to be established in great detail:(1)single-cell/single-nucleus RNA-seq technology establishes high-throughput transcriptomic references,covering all major transcriptomic cell types;(2)patch-seq links the morphological and electrophysiological features to the transcriptomic reference;(3)multicell patch-clamp delineates the principles of local connectivity.Here,we review the applications of these technologies in the primate neocortex and discuss the current advances and tentative gaps for a comprehensive understanding of the primate neocortex.

关 键 词：NEURON Brain atlas Patch-seq CONNECTIVITY PRIMATE 

分 类 号：R741[医药卫生—神经病学与精神病学]