体外模拟消化过程中青川核桃壳和分心木抗氧化成分及活性的变化  

Effects of in vitro simulated digestion on antioxidant components and activities of walnut shell and distraction wood

作　　者：杜昕 张新悦 王芳 罗琴[1] 谢庆 赵英琼 刘雨潇 DU Xin;ZHANG Xinyue;WANG Fang;LUO Qin;XIE Qing;ZHAO Yingqiong;LIU Yuxiao(College of Chemistry and Life Sciences,Chengdu Normal University,Chengdu,Sichuan 611130,China;Institute of Food Fermentation,Chengdu Normal University,Chengdu,Sichuan 611130,China;Sichuan Provincial Key Laboratory for Development and Utilization of Characteristic Horticultural Biological Resources,Chengdu,Sichuan 611130,China)

机构地区：[1]成都师范学院化学与生命科学学院,四川成都611130 [2]成都师范学院食品发酵研究所,四川成都611130 [3]特色园艺生物资源开发与利用四川省高等学校重点实验室,四川成都611130

出　　处：《食品与机械》2024年第5期154-160,共7页Food and Machinery

基　　金：教育部大学生创新创业训练计划支持项目(编号:S2022143891223,S202314389191,S202314389128)。

摘　　要：目的:充分利用核桃副产物资源。方法:采用超声辅助乙醇提取核桃壳和分心木抗氧化成分,探究其提取物在体外模拟消化过程中抗氧化成分(总酚、总黄酮含量)和抗氧化性(DPPH自由基清除活性、ABTS自由基清除活性、羟自由基清除活性,FRAP和Fe^(2+)螯合力)的变化规律,并分析其相关性。结果:分心木的总酚、总黄酮含量、抗氧化活性始终高于核桃壳的(P<0.05);经体外模拟消化后,分心木和核桃壳中总酮、总酚含量逐渐降低(P<0.05),DPPH自由基清除力、ABTS自由基清除力和FRAP抗氧化能力为口腔>胃>肠(P<0.05),与总酚、总黄酮含量变化趋势一致。除Fe^(2+)螯合外,核桃壳和分心木提取物消化液的抗氧化性与总酚和总黄酮含量呈显著正相关(P<0.05)。结论:核桃壳和分心木均具有一定抗氧化活性,且分心木的抗氧化活性优于核桃壳,有望作为天然抗氧化功能食品或抗氧化剂的原料来源。Objective:This study aimed to make full use of walnut by-products.Methods:Ultrasonic-assisted ethanol extraction method was used to extract the antioxidant components of walnut shell and distraction wood.The changes of antioxidant components(total phenols and total flavonoids)and antioxidant activities(DPPH,ABTS,hydroxyl radical scavenging ability,Iron reduction antioxidant capacity-FRAP and ferrous ion chelating activity)of walnut by-products extract during the simulated digestion were studied in vitro,and their correlation was analyzed.Results:The total phenols(TPC),total flavonoids(TFC)content,and antioxidant activity of distraction wood were always higher than those of walnut shells(P<0.05).After simulated in vitro digestion,the TPC and TFC of both walnut shell and distraction wood decreased gradually(P<0.05).The antioxidant capacity of FRAP and DPPH,ABTS+free radical scavenging capacity were approximately as follows:oral>stomach>intestine(P<0.05),a trend consistent with the changes of TPC and TFC.Except for Fe^(2+)chelating activity,the antioxidant capacity of the digestive juices showed a significant positive correlation with TPC and TFC(P<0.05).Conclusion:Distraction wood extract has an impressive antioxidant activity which was superior to walnut shell extract and was expected to be used as a natural antioxidant functional food or raw material source of antioxidants.

关 键 词：核桃壳 分心木 体外模拟消化 抗氧化活性 

分 类 号：TS209[轻工技术与工程—食品科学]