超声波处理与纤维素酶协同提取山楂总多酚的工艺优化研究  

Optimization of Ultrasonic Wave and Cellulase Synergistic Extraction Technology of Total Polyphenols from Crataegi Fructus

作　　者：周志红[1] 赵昕[1] 胡勤[1] 肖明明 Zhou Zhihong;Zhao Xin;Hu Qin;Xiao Mingming(Department of Pharmacy,Hu'nan Children's Hospital,Hu'nan,410007)

机构地区：[1]湖南省儿童医院药学部,湖南410007

出　　处：《当代化工研究》2024年第11期152-155,共4页Modern Chemical Research

基　　金：湖南省自然科学基金青年基金“基于灰色关联度法的儿童增免膏的谱效关系研究及对儿童反复呼吸道感染的增免作用”(项目编号:2021JJ40279);湖南省卫生健康委一般指导项目“补肺健脾增免膏对小鼠黏膜免疫系统SIgA的影响及谱效关系研究”(项目编号:202113011086)。

摘　　要：目的:优化超声波处理与纤维素酶协同提取山楂总多酚的工艺条件。方法:以总多酚得率为指标,采用单因素实验和响应面法优化山楂总多酚的提取工艺。结果:最佳工艺条件为:纤维素酶添加量5%、乙醇体积分数62%、超声温度58℃、液料比40:1(mL/g)、超声功率240 W、超声时间30 min。在此条件下,山楂总多酚的得率为(80.46±1.62)mg/g。结论:该优化工艺简便、可行,可为山楂多酚的深入研究开发提供科学依据。Objective:The present study aimed to optimize the ultrasonic wave and cellulase synergistic extraction technology of total polyphenols from Crataegi fructus.Methods:Taking the extraction yield of total polyphenols as the metric,the extraction process of total polyphenols from Crataegi fructus was optimized based on the single factor test and the response surface methods.Results:The optimum extraction parameters were the amount of cellulase added 5%,ethanol volume fraction 62%,ultrasonic temperature 58℃,liquid-material ratio 40:1(mL/g),ultrasonic power 240 W and ultrasonic time 30 min.The total polyphenols extraction yield of Crataegi fructus was(80.46±1.62)mg/g under these conditions.Conclusions:This optimized extraction process is simple and feasible,which provides scientific evidence for the further study and exploit of polyphenols from Crataegi fructus.

关 键 词：山楂总多酚 超声波处理 纤维素酶 响应面法 

分 类 号：TS201.2[轻工技术与工程—食品科学]