茶叶籽粕蛋白酶解物的抗氧化及抑制α-葡萄糖苷酶活性研究  

Antioxidative and Anti-α-glucosidase Activities of Enzymatically Hydrolyzed Tea Seed Protein

作　　者：陈巧玲 卢学慧 王弛 赵峰 CHEN Qiao-ling;LU Xue-hui;WANG Chi;ZHAO Feng(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China)

机构地区：[1]福建中医药大学药学院,福建福州350122

出　　处：《茶叶学报》2024年第2期64-71,共8页Acta Tea Sinica

基　　金：福建中医药大学引进人才科研启动项目(2801/701190097)。

摘　　要：【目的】提取茶叶籽粕蛋白,并比较其经不同蛋白酶酶解后的抗氧化活性和α-葡萄糖苷酶抑制率,进而评估将其作为新型生物活性肽生产原料的可行性。【方法】首先通过碱提酸沉法获得茶叶籽粕蛋白粗提物,再分别以碱性蛋白酶、中性蛋白酶和木瓜蛋白酶进行酶解;比较各酶解物的DPPH自由基清除率、ABTS自由基清除率、总还原力和α-葡萄糖苷酶抑制率的差异。【结果】采用碱提酸沉法(0.1 mol·L^(-1) NaOH提取、1 mol·L^(-1) HCl调整pH至3.5)制备的茶叶籽粕蛋白粗提物,其得率为7.7%,蛋白纯度27.6%。酶解物活性评价结果显示,碱性蛋白酶酶解物对DPPH自由基清除率最高,其IC_(50)(半数抑制浓度)为80.17μg·mL^(-1);对ABTS自由基清除率最高,其IC_(50)为123.79μg·mL^(-1);总还原力最强,为0.458;浓度为1 mg·mL^(-1)时对α-葡萄糖苷酶抑制率较强,为93.12%。【结论】采用碱性蛋白酶酶解茶叶籽粕蛋白粗提物,其酶解物具有开发成抗氧化和抑制α-葡萄糖苷酶生物活性肽的潜力。【Objective】Antioxidative and anti-α-glucosidase activities of enzymatically hydrolyzed protein extracted from tea seed meal were determined for the development of a functional peptide product.【Method】Camellia sinensis seed meal was alkaline-extracted using 0.1 mol·L^(-1) NaOH and followed by acid precipitation with 1 mol·L^(-1) HCl at pH 3.5 to obtain crude protein which was subsequently enzymatically hydrolyzed.The hydrolysates produced by applying alcalase,a neutral protease,or papain were tested for DPPH and ABTS free radical scavenging capacities as well as reducing power and α-glucosidase inhibition.【Result】The tea seed protein extraction process yielded 7.7%solids that contained 27.6% protein.Among the 3 proteases,alacase delivered a hydrolysate with the highest IC50 of 80.17μg·mL^(-1) in scavenging DPPH free radicals and an IC_(50) of 123.79μg·mL^(-1) in scavenging ABTS free radicals.The hydrolysate also showed the greatest total reducing power of 0.458 andα-glucosidase inhibition rate of 93.12% at an applied concentration of 1 mg·mL^(-1).【Conclusion】The alacase-hydrolyzed tea seed protein exhibited significant in vitro free radical scavenging and anti-α-glucosidase activities of a bioactive peptide.

关 键 词：茶叶籽粕蛋白 蛋白酶 酶解物 抗氧化 Α-葡萄糖苷酶 

分 类 号：TS272[农业科学—茶叶生产加工]