硫酸亚铁铵-分光光度法测定面粉中过氧化苯甲酰含量的研究  

Determination of Benzoyl Oxide Content in Flour by Ammonium Ferrous Sulfate and Spectrophotometry

作　　者：何静[1] 杨英 罗玲 郭佳[2] 邹元 黄丽玟 HE Jing;YANG Ying;LUO Ling;GUO Jia;ZOU Yuan;HUANG Liwen(Sichuan Vocational College of Chemical Technology,Luzhou 646000,China;Luzhou Laojiao Co.,Ltd.,Luzhou 646000,China)

机构地区：[1]四川化工职业技术学院,四川泸州646000 [2]泸州老窖股份有限公司,四川泸州646000

出　　处：《食品安全导刊》2024年第16期124-127,共4页China Food Safety Magazine

基　　金：天然产物与功能性食品开发研究泸州市重点实验室项目“青蒿植物饮品的研制”(HYGNSP-2206-B)。

摘　　要：采用硫酸亚铁铵-可见分光光度计测定面粉中过氧化苯甲酰的含量,并建立测定面粉中过氧化苯甲酰含量的方法。分光光度法标准曲线的回归方程为A=0.247 2C+0.001 9,相关系数R^(2)=0.999,显著性检验P<0.01,重复性实验的相对标准偏差为0.83%(<1%),加标实验的回收率为98.62%,测量的最低浓度为0.5 mg·kg^(-1),实验误差与国家标准《小麦粉中过氧化苯甲酰的测定 高效液相色谱法》(GB/T 22325—2008)中高效液相色谱法的相对误差在-1.5%~1.5%。该方法所建立的样品溶液吸光度与浓度之间呈正相关关系,所建立的测试方法具有较高的实验精度、重复性和可信性。The content of benzoyl peroxide in flour was determined using a ferrous ammonium sulfate visible spectrophotometer,and establishing a method for determining the content of benzoyl peroxide in flour.The regression equation for the standard curve of spectrophotometry is A=0.2472C+0.0019,correlation coefficient R^(2)=0.999,significance test P<0.01,relative coefficient of variation of repeatability experiment is 0.83%,less than 1%,recovery rate of spiking experiment is 98.62%,the minimum concentration measured is 0.5 mg·kg^(-1),and the relative error between the experimental error and the high-performance liquid chromatography method in the national standard GB/T 22325—2008 is-1.5%~1.5%.The absorbance of the sample solution established under this method shows a positive linear relationship with concentration,and the established testing method has high experimental accuracy,repeatability,and credibility.

关 键 词：过氧化苯甲酰 面粉 分光光度法 标准曲线 准确度 

分 类 号：TS211.7[轻工技术与工程—粮食、油脂及植物蛋白工程] O657.3[轻工技术与工程—食品科学与工程]