酶解提取肉鸽血液中血红素铁的工艺优化  

Optimization of the Enzymatic Extraction of Heme Iron from Pigeon Blood

作　　者：张远红 张纬嘉 李滢 陈丽婷 陈伟波 曾晓房[1] ZHANG Yuanhong;ZHANG Weijia;LI Ying;CHEN Liting;CHEN Weibo;ZENG Xiaofang(Guangdong Provincial Key Laboratory of Lingnan Specialty Food Science and Technology,College of Light Industry and Food Sciences,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,China;Meizhou Golden Green Modern Agriculture Development Co.Ltd.,Meizhou 514500,China)

机构地区：[1]仲恺农业工程学院轻工食品学院,广东省岭南特色食品科学与技术重点实验室,广东广州510225 [2]梅州市金绿现代农业发展有限公司,广东梅州514500

出　　处：《肉类研究》2024年第4期17-22,共6页Meat Research

基　　金：梅州市科技计划项目(2023A0304001)。

摘　　要：以肉鸽血液为原料,采用木瓜蛋白酶、中性蛋白酶、碱性蛋白酶、风味蛋白酶和复合蛋白酶对其中的血红素铁进行提取。以血红素铁含量为主要评价指标,筛选出木瓜蛋白酶和复合蛋白酶进行复配酶解,以血红素铁含量和水解度为评价指标,通过单因素和正交试验优化确定肉鸽血液血红素铁的最佳提取工艺,结果表明:木瓜蛋白酶、复合蛋白酶配比2∶1、pH 7.0、温度50℃、血水比1∶2、总加酶量8000 U/g、酶解时间3 h工艺下,肉鸽血液中血红素铁的提取量达到95.43μg/mL,与单酶提取法相比血红素铁得率显著提高,可实现肉鸽血液的高附加值利用。In this research,heme iron from meat-type pigeon blood was extracted by enzymatic hydrolysis using papain,neutral protease,alkaline protease,flavourzyme or protamex.Based on heme iron content,papain and protamex were selected for dual-enzymatic hydrolysis.Employing one-factor-at-a-time(OFAT)method and orthogonal array design(OAD)with heme iron content and hydrolysis degree as evaluation indicators,the optimum extraction conditions were determined as follows:papain-to-protamex ratio 2:1,pH 7.0,temperature 50℃,blood-to-water ratio of 1:2,enzyme dosage 8000 U/g,and hydrolysis time 3 h.Under these conditions,the extraction yield of heme iron was 95.43μg/mL,which was significantly increased compared with that obtained using single enzymes.

关 键 词：肉鸽血液 血红素铁 酶法提取 工艺优化 

分 类 号：TS251.93[轻工技术与工程—农产品加工及贮藏工程]