基于COI和16S rRNA基因序列的鱼胶基原鱼种DNA条形码鉴定  

DNA Barcoding identification on original species of isinglass through COI and 16S rRNA sequences

作　　者：黄蓬英 罗莹 曾琪 赵光宇 杜瑞珠 苏丹 徐敦明 HUANG Peng-Ying;LUO Ying;ZENG Qi;ZHAO Guang-Yu;DU Rui-Zhu;SU Dan;XU Dun-Ming(Xiamen Customs Technology Center,Xiamen 361026,China;Guangdong Chaozhiyuan Biotechnology Co.,Ltd.,Shantou 515000,China;Fujian Fangjiapuzi Co.,Ltd.,Putian 351100,China)

机构地区：[1]厦门海关技术中心,厦门361026 [2]广东潮之源生物科技有限公司,汕头515000 [3]福建省方家铺子有限公司,莆田351100

出　　处：《食品安全质量检测学报》2024年第9期69-77,共9页Journal of Food Safety and Quality

基　　金：国家重点研发计划专项(2023YFF1104900)。

摘　　要：目的建立基于COI和16S rRNA基因序列的DNA条形码鱼胶基原鱼种鉴定方法。方法以汕头、厦门、莆田等地采集的28份鱼胶为研究对象,利用形态归类法对28份样品进行观察,再提取基因组DNA,分别利用6对引物扩增COI和16S rRNA基因序列并双向测序。聚合酶链式反应(polymerasechain reaction,PCR)产物测序分析后,输入Genbank中进行BLAST分析比对,再利用最大似然法(maximum likelihood,ML)进行系统发育分析。结果采集的28份鱼胶样品可分为11种类型,主要有萝卜型、“Y”字型、心型、纸片型和琵琶型等。BLAST分析比对共鉴定出26份鱼胶样品基原鱼种,隶属于5目6科9属12种。系统发育分析表明不同种类的鱼胶处于不同分支中。结论该方法可用于鱼胶基原鱼种的快速分子鉴定,也为鱼胶的真伪鉴定及溯源提供理论依据。Objective To establish a method for the identification of the origin of isinglass by DNA barcoding.Methods A total of 28 experiment samples of isinglass were collected from Shantou,Xiamen and Putian.Samples were observed,genomic DNA were extracted from isinglass and PCR were performed with the COI and 16S rRNA primers.The amplified sequences were aligned in the Genbank database,ML-tree was built as well.Results All experiment isinglass samples were classified for 11 morphological types,including carrot-shaped,“Y”type,heart-shaped,piece of paper type and pipa type.Total 26 samples could be successfully distinguished by gene COI and 16S rRNA and belong to 5 orders 6 families 9 genes 12 species.The phylogenetic tree revealed that different types of isinglass were in different branches.Conclusion The COI and 16S rRNA sequences can be used to distinguish the origin of isinglass and they are efficient for tracing the origin species of isinglass.

关 键 词：鱼胶 形态归类 COI 16S rRNA 基原鉴定 

分 类 号：TS254.5[轻工技术与工程—水产品加工及贮藏工程] R155.5[轻工技术与工程—食品科学与工程]