高效液相色谱法同时检测特殊用途饮料中常见的咖啡因等6种食品添加剂  

Simultaneous Detection of Six Common Food Additives(Caffeine tec.)in Special Purpose Beverages Using High-performance Liquid Chromatography

作　　者：凌玲 周雨[1] 袁媛[1] 安娜[1] LING Ling;ZHOU Yu;YUAN Yuan;AN Na(Shijingshan District Center for Disease Control and Provention,Beijing 10043,China)

机构地区：[1]北京市石景山区疾病预防控制中心理化科,北京100043

出　　处：《饮料工业》2024年第3期30-34,共5页Beverage Industry

摘　　要：目的建立使用高效液相色谱仪同时检测特殊用途饮料中的咖啡因、糖精钠、安赛蜜、柠檬黄、胭脂红、日落黄等6种食品添加剂的方法。方法仪器使用C18色谱柱,流动相采用甲醇与0.02mol/L乙酸铵溶液(不需要调节pH值),经梯度洗脱,通过四个检测波长通道同时采集色谱图和光谱图进行检测分析。结果上述6种食品添加剂线性范围在1.00 mg/L~100.0mg/L,相关系数均在0.9999以上,回收率为91.0%~108.2%。结论本方法操作简单、快速,可用于同时检测特殊用途饮料中的咖啡因等6种食品添加剂。Objective To establish a method using high-performance liquid chromatography(HPLC)for the simultaneous detection of six food additives,namely caffeine,sodium saccharin,aspartame,tartrazine,erythrosine,and sunset yellow,in functional beverages.Methods The instrument employed a C18 chromatographic column and a mobile phase composed of methanol and a 0.02mol/L solution of ammonium acetate(pH not adjusted).The compounds were eluted by gradient elution,and chromatograms and spectra were simultaneously collected using four detection wavelength channels.Results The linear range for the six food additives was 1.00 to 100.0mg/L,with correlation coefficients all above 0.9999,and recovery ratios ranging from 91.0%to 108.2%.Conclusion This method is simple,rapid,and suitable for the simultaneous detection of caffeine and the other five other food additives in functional beverages.

关 键 词：高效液相色谱法 特殊用途饮料 咖啡因 合成着色剂 同时检测 

分 类 号：TS207.3[轻工技术与工程—食品科学]