樟树籽仁油的90 d亚慢性经口毒性评价  

Evaluation of 90-day subchronic oral toxicity of Cinnamomum camphora seed kernel oil

作　　者：彭婷[1,2,3] 梁丽军 曾哲灵 李美娜[1,2,6] 鄢祥辉 夏佳恒[1,2,5] 余平 万冬满 文学方 PENG Ting;LIANG Lijun;ZENG Zheling;LI Meina;YAN Xianghui;XIA Jiaheng;YU Ping;WAN Dongman;WEN Xuefang(State Key Laboratory of Food Science and Resources,Nanchang University,Nanchang 330047,China;Jiangxi Province Key Laboratory of Edible and Medicinal Resources Exploitation,Nanchang University,Nanchang 330031,China;School of Food Science and Technology,Nanchang University,Nanchang 330031,China;PureCircle(Jiangxi)Co.,Ltd.,Ganzhou 341108,Jiangxi,China;School of Chemistry and Chemical Engineering,Nanchang University,Nanchang 330031,China;School of Resources and Environment,Nanchang University,Nanchang 330031,China;Institute of Applied Chemistry,Jiangxi Academy of Sciences,Nanchang 330096,China;Institute of Traditional Chinese Medicine Health Industry,China Academy of Chinese Medical Sciences,Nanchang 330115,China)

机构地区：[1]南昌大学食品科学与资源挖掘全国重点实验室,南昌330047 [2]江西省药食同源植物资源高值化利用重点实验室,南昌330031 [3]南昌大学食品学院,南昌330031 [4]谱赛科(江西)生物技术有限公司,江西赣州341108 [5]南昌大学化学化工学院,南昌330031 [6]南昌大学资源与环境学院,南昌330031 [7]江西省科学院应用化学研究所,南昌330096 [8]中国中医科学院中医药健康产业研究所,南昌330115

出　　处：《中国油脂》2024年第7期83-89,100,共8页China Oils and Fats

基　　金：国家国际科技合作专项项目(2011DFA32770);国家自然科学基金项目(31701651);国家自然科学基金项目(32060516);江西省科技支撑计划重大项目(20143ACG70015);南昌大学食品与技术国家重点实验室自由探索课题(SKLF-ZZB-202135);南昌大学食品与技术国家重点实验室自由探索课题(SKLF-ZZB-201916);江西省重点研发计划项目(20212BBF63035);江西省科学院省级包干制项目(2022YSBG21021);江西省科学院省级科研院基础研究项目(2022YJC2016)。

摘　　要：为评价樟树籽仁油的食用安全性,以4.0、2.0、1.0、0.0 mL/kg剂量的樟树籽仁油对4组SPF级SD大鼠(20只/组、雌雄各半)连续灌胃90 d,研究樟树籽仁油的亚慢性经口毒性,探明其剂量-反应关系,毒作用靶器官和可逆性。结果表明:90 d经口毒性试验期间,大鼠的活动和生长均正常;除高剂量组雌、雄性大鼠的血糖显著低于对照组外(p<0.05),各剂量组雌、雄性大鼠的体质量及其增量、进食量及食物利用率、脏体比、血常规、血生化、血电解质和尿液等指标与对照组均无显著性差异(p>0.05);病理检查未见樟树籽仁油对大鼠的作用靶器官产生有意义的病理变化。综上,樟树籽仁油对大鼠生长和生理的影响无剂量-反应关系,对大鼠的作用靶器官无毒害作用且无剂量-反应关系,樟树籽仁油无亚慢性经口毒性,其未观察到毒效应的剂量(NOAEL)大于4.0 mL/kg,食用安全性高。In order to evaluate the edible safety of Cinnamomum camphora seed kernel oil(CCSKO),four groups of specific pathogen-free(SPF)Sprague-Dawley(SD)rats(20 rats/group,half male and half female)were given CCSKO at doses of 4.0,2.0,1.0 mL/kg and 0.0 mL/kg for 90 d by gavage.The subchronic oral toxicity of CCSKO was studied,and its dose-response relationship,toxic target organs and reversibility were explored.The results showed that during the 90-day oral toxicity test,the activity and growth of the rats were normal.There were no significant differences in body weight,weight gain,food intake,food utilization rate,visceral-body ratio,blood routine,blood biochemistry,blood electrolytes and urine between the dose groups and control group(p>0.05),except that the blood glucose of the female and male rats in the high dose group was significantly lower than that in the control group(p<0.05).No significant pathological changes were found in the target organs of CCSKO.In conclusion,CCSKO has no dose-response relationship on the growth and physiology of rats,has no toxic effect on the target organs of rats,and has no dose-response relationship.CCSKO has no subchronic oral toxicity,and its no observed adverse effect level(NOAEL)is higher than 4.0 mL/kg,and it has high safety for consumption.

关 键 词：樟树籽仁油 毒理学 90 d亚慢性经口毒性 食用安全性 

分 类 号：TS222[轻工技术与工程—粮食、油脂及植物蛋白工程]