QuEChERS结合气相色谱-串联质谱法测定啤酒花中45种农药残留  

Determination of 45 Pesticide Residues in Hops by QuEChERS Combined with Gas Chromatography-Tandem Mass Spectrometry

作　　者：刘家新 曾婷 殷卓均 杜业刚[1] 朱雨田[1] 郑彦婕[1] 张世伟[1] 罗淙岚 LIU Jiaxin;ZENG Ting;YIN Zhuojun;DU Yegang;ZHU Yutian;ZHENG Yanjie;ZHANG Shiwei;LUO Conglan(Shenzhen Academy of Metrology and Quality Inspection,Shenzhen 518100,China;South China Agricultural University,Guangzhou 510000,China)

机构地区：[1]深圳市计量质量检测研究院,广东深圳518100 [2]华南农业大学,广东广州510000

出　　处：《食品科技》2024年第5期334-341,共8页Food Science and Technology

基　　金：深圳市可持续发展专项(KCXFZ20230731092859013)。

摘　　要：文章建立一种快速分散固相萃取(Quick,easy,cheap,effective,rugged,and safe,QuEChERS)技术结合气质联用仪(Gas chromatography-mass spectrometry,GC-MS/MS)测定啤酒花中45种农药残留的分析方法。样品经乙酸乙腈提取,QuEChERS和Z-Sep组合净化,通过GC-MS/MS检测,用外标法对农药残留进行定量分析。该方法中45种农药在0.010~0.500 mg/L范围内均具有良好的线性相关性,相关系数(R^(2))均大于0.998。在0.03、0.05、0.10 mg/kg 3个不同质量浓度水平下进行加标试验,回收率为66.7%~106.0%,相对标准偏差(n=6)为0.41%~8.95%,定限量为0.01 mg/kg。该方法操作简便、高效快速,适用于啤酒花中农药多残留的快速筛查。An analysis method was established for the determination of 45 kinds of pesticide residues in hops by quick,easy,cheap,effective,rugged,and safe (QuEChERS) combined with gas chromatography-mass spectrometry (GC-MS/MS).The samples were extracted with acetonitrile acetate,and then clean-up was carried out by QuEChERS and Z-Sep,develop a external standards quantitative method for pesticide residues through GC-MS/MS.The 45 pesticides in this method had a good linear correlation in the range of 0.010–0.500 mg/L,the correlation coefficients (R~2) greater than 0.998.In three different concentration levels of 0.03 mg/kg,0.05 mg/kg and 0.10 mg/kg,the average recoveries were 66.7%–106.0% with the relative standard deviations (n=6) between 0.41% and 8.95%,and limit of quantitative was 0.01 mg/kg.The method is simple,rapid and accurate,which is suitable for determination of pesticide residues in hops.

关 键 词：啤酒花 气相色谱-质谱法 农药残留 QUECHERS 

分 类 号：TS207.53[轻工技术与工程—食品科学]