碱性及复合蛋白酶复配水解对脱脂牛乳致敏性的影响  

The Effects of Alcalase and Protamex Compound Enzymatic Hydrolysis on the Allergenicity of Skimmed Milk

作　　者：钱冠林 孙敬 杨慧[1] 梁肖娜 岳喜庆[1] 郑艳[1] Qian Guanlin;Sun Jing;Yang Hui;Liang Xiaona;Yue Xiqing;Zheng Yan(College of Food Science,Shenyang Agricultural University,Shenyang 110000)

机构地区：[1]沈阳农业大学食品学院,沈阳110000

出　　处：《中国食品学报》2024年第6期97-106,共10页Journal of Chinese Institute Of Food Science and Technology

基　　金：“十三五”国家重点研发计划重点专项(2018YFC1604302-03)。

摘　　要：牛乳中致敏蛋白在一定程度上限制了其在婴幼儿配方奶粉中的使用。以脱脂牛乳为原料,以水解度、脱脂牛乳IgG抑制率、过敏患者血清IgE抑制率为指标,研究碱性、复合蛋白酶复配处理对其致敏性的影响。结果表明,复配酶的酶活与底物比(E/S)为6000 U/g,碱性蛋白酶与复合蛋白酶质量比为3∶1,酶解时间60 min时,脱脂牛乳的水解度为9.21%,对脱脂牛乳IgG及过敏患者血清IgE抗体抑制率分别为86.94%与43.46%,显著高于单一蛋白酶(P<0.05)的处理效果。研究结果为以脱脂牛乳为婴幼儿奶粉基料的开发提供了重要参考。The presence of allergenic proteins in cow milk limits its use as infant formula to a certain extent.Skimmed milk was used as raw material,the degree of hydrolysis,the IgG inhibition rate of skimmed milk and IgE inhibition rate of patients were used as indicators and the effects of alcalase and protamex compound treatment on its sensitization were investigated.The results showed that the most suitable conditions for the enzymatic hydrolysis were the substrate ratio(E/S)of alcalase and protamex was 6000 U/g,the composite ratio for alcalase and protamex was 3∶1,and the hydrolysis time was 60 min,and the inhibition rate of the compound enzyme on skimmed milk IgG and IgE antibodies were significantly higher than that effect of the single protease treatment(P<0.05),which were 86.94% and 43.46%,respectively,indicating that the compound enzyme is an effective method to reduce the allergenicity of skimmed milk.

关 键 词：脱脂牛乳 复配酶解 致敏性 ELISA 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]