高效液相色谱法同时检测葡萄酒中11种人工合成着色剂  

Simultaneous determination of 11 synthetic colorants in wine by HPLC

作　　者：李广[1] 李琪[1] 顾敏[1] 张锋[1] LI Guang;LI Qi;GU Min;ZHANG Feng(Analysis and Research Center of Gansu Province,Lanzhou 730000,China)

机构地区：[1]甘肃省分析测试中心,甘肃兰州730000

出　　处：《中国酿造》2024年第7期246-250,共5页China Brewing

基　　金：甘肃省属科研院所创新能力建设专项(21JR7RA765)。

摘　　要：为快速识别葡萄酒中是否添加人工合成着色剂并准确定量,该实验样品采用乙酸铵溶液稀释、8000 r/min离心后直接过滤法进行前处理,梯度洗脱、Zorbax SB-C_(18)柱分离、多波长检测(288 nm、425 nm、515 nm、620 nm),建立了高效液相色谱法(HPLC)同时检测葡萄酒中11种人工合成着色剂的方法。结果表明,该方法前处理简单,各着色剂最大吸收波长检测无杂质干扰、灵敏度高、检出限(0.05 mg/L)、定量限(0.15 mg/L)低,在质量浓度0.20~50 mg/L范围内线性关系良好(R^(2)≥0.9994),方法精密度试验结果的相对标准偏差(RSD)为1.86%~2.61%,加标回收率为93.0%~103.0%。适用于葡萄酒中人工合成着色剂的快速、准确检测。Aiming to quickly identify whether synthetic colorants are added to wine and accurately quantify,a HPLC method was established to simultaneously detect 11 kinds of synthetic colorants in wine.The experimental samples were diluted with ammonium acetate solution,filtered directly after centrifugation at 8000 r/min,separated with Zorbax SB-C_(18) column by gradient elution,and detected by multi-wavelength(288 nm,425 nm,515 nm,620 nm).The results shown that the method was characterized by simple pre-treatment,no interference from impurities under the maximum absorption wavelength of each colorants,high sensitivity,low detection limit(0.05 mg/L),low limit of quantitation(0.15 mg/L),and good linear relationship(R^(2)≥0.9994)in the range of 0.20-50 mg/L.The relative standard deviation(RSD)of precision tests results was 1.86%-2.61%,and the recovery rates were 93.0%-103.0%.The method was suitable for rapid and accurate detection of synthetic colorants in wine.

关 键 词：人工合成着色剂 葡萄酒 直接过滤 检测 高效液相色谱 

分 类 号：TS262.6[轻工技术与工程—发酵工程] O657.63[轻工技术与工程—食品科学与工程]