Sgo1 interacts with CENP-A to guide accurate chromosome segregation in mitosis  

作　　者：Fengge Wu Hameed Akbar Chunyue Wang Xiao Yuan Zhen Dou McKay Mullen Liwen Niu Liang Zhang Jianye Zang Zhikai Wang Xuebiao Yao Xiaoyu Song Xing Liu 

机构地区：[1]MOE Key Laboratory for Cellular Dynamics,Center for Advanced Interdisciplinary Science and Biomedicine of Institute of Health and Medicine(IHM),University of Science and Technology of China School of Life Sciences,Hefei 230026,China [2]Anhui Key Laboratory for Cellular Dynamics and Chemical Biology,Hefei National Center for Cross-disciplinary Sciences,Hefei 230027,China [3]Keck Center for Cellular Dynamics,Morehouse School of Medicine,Atlanta,GA 30310,USA

出　　处：《Journal of Molecular Cell Biology》2023年第10期26-38,共13页分子细胞生物学报（英文版）

基　　金：supported by grants from the Ministry of Science and Technology of China(2022YFA1303100,2022YFA0806800,2022YFA1302700,and 2017YFA0503600);the National Natural Science Foundation of China(32090040,92254302,92153302,32170733,31621002,and 22177106);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB19040000 and XDB37010105);the Ministry of Education(IRT_17R102,20113402130010,and YD2070006001).

摘　　要：Shugoshin-1(Sgo1)is necessary for maintaining sister centromere cohesion and ensuring accurate chromosome segregation during mitosis.It has been reported that the localization of Sgo1 at the centromere is dependent on Bub1-mediated phosphorylation of histone H2A at T120.However,it remains uncertain whether other centromeric proteins play a role in regulating the localization and function of Sgo1 during mitosis.Here,we show that CENP-A interacts with Sgo1 and determines the localization of Sgo1 to the centromere during mitosis.Further biochemical characterization revealed that lysine and arginine residues in the C-terminal domain of Sgo1 are critical for binding CENP-A.Interestingly,the replacement of these basic amino acids with acidic amino acids perturbed the localization of Sgo1 and Aurora B to the centromere,resulting in aberrant chromosome segregation and premature chromatid separation.Taken together,these findings reveal a previously unrecognized but direct link between Sgo1 and CENP-A in centromere plasticity control and illustrate how the Sgo1–CENP-A interaction guides accurate cell division.

关 键 词：MITOSIS CENTROMERE KINETOCHORE Sgo1 CENP-A Aurora B 

分 类 号：Q2[生物学—细胞生物学]