陕西茯茶抗氧化成分的提取及其定量构效关系(QSAR)研究  

Extraction of antioxidant compounds from Shaanxi Fu tea and its quantitative structure-activity relationship(QSAR)

作　　者：周彩华 梁艳 王冉 王晨祥 曹博文 孙越赟 ZHOU Caihua;LIANG Yan;WANG Ran;WANG Chenxiang;CAO Bowen;SUN Yueyun(Xianyang Normal University,Xianyang 712000;Xianyang Jingwei Fucha Limited Company,Xianyang 712044;Shaanxi Province Modern Agricultural Park Service Center,Xi'an 710003;Shanghai Institute of Technology,Shanghai 200093)

机构地区：[1]咸阳师范学院,咸阳712000 [2]咸阳泾渭茯茶有限公司,咸阳712044 [3]陕西省现代农业园区服务中心,西安710003 [4]上海理工大学,上海200093

出　　处：《中国食品添加剂》2024年第7期23-32,共10页China Food Additives

基　　金：咸阳科技局项目(2022KJJ03);宁夏科技厅项目(2022AAC0339);咸阳市泾阳茯茶发展中心项目(2022XSYH186);大学生创业创新项目(S202210722075)。

摘　　要：以陕西茯砖黑毛茶为原料,基于溶剂萃取法的单因素实验对有效成分进行提取,通过正交实验测定陕西茯茶提取物对DPPH和ABTS+自由基的清除率来反映其有效成分的抗氧化能力。研究结果表明利用乙醇-水溶液萃取法获得的提取物具有较强的抗氧化成分,抗氧化成分提取的最佳条件为:40%的乙醇溶液,提取温度45℃、提取时间45 min、料液比为1∶60。研究发现该有效成分对DPPH的清除率高达85%,高于对ABTS+自由基的清除率(约67%)。对茯茶素分子的化学物理属性与抗氧化的关联性进行了定量构效关系的研究,揭示出含有酚羟基的茯茶素分子的抗氧化性与分子中氢键给体的数量存在线性关系。总之,本文对陕西茯茶中多酚的提取工艺以及抗氧化性能的定量构效关系(QSAR)的研究可以为陕西茯茶抗氧化产品的开发提供理论依据。Using Shaanxi Fu brick tea as the raw material,the active ingredients were extracted based on a solvent extraction method through single-factor experiments.The antioxidant capacity of the effective components was reflected by the clearance rate of DPPH and ABTS+free radicals in Shaanxi Fu tea extract,determined through orthogonal experiments.The findings revealed that the extract obtained using an ethanol-water solution extraction method possessed high antioxidant capacity.The optimal conditions for extracting antioxidant components are as follows:a 40% ethanol solution,extraction time of 45℃,extraction time of 45 minutes,and a solid-to-liquid ratio of 1∶60.The extract showed an 85%clearance rate for DPPH radicals,which was higher than the clearance rate of 67% for ABTS+radicals.A quantitative structure-activity relationship study was conducted on the chemical and physical properties of the catechin molecules to elucidate the correlation between these properties and antioxidant activity,revealing a linear relationship between the antioxidant properties of catechin molecules containing phenolic hydroxyl groups and the number of hydrogen bond acceptors in the molecule.This research on the extraction and QSAR of antioxidant components in Shaanxi Fu tea lays a theoretical foundation for developing antioxidant products from this tea.

关 键 词：溶剂萃取 抗氧化性 茯砖茶 定量构效关系 

分 类 号：TS202.1[轻工技术与工程—食品科学]