凯氏定氮法测定杏鲍菇中蛋白质的条件优化研究  

Study on Optimum Conditions for Determination of Protein in Pleurotus eryngii by Kjeldahl Nitrogen Determination Method

作　　者：阳焱红 朱斐淇 曾静 莫寒 杨期辉 刘会敬 YANG Yanhong;ZHU Feiqi;ZENG Jing;MO Han;YANG Qihui;LIU Huijing(Department of Life Sciences,Nanyue College of Hengyang Normal University,Hengyang 421000,China;College of Life Sciences,Hengyang Normal University,Hengyang 421000,China;Hunan Key Laboratory for Conservation and Utilization of Biological Resources in the Nanyue Mountainous Region,Hengyang 421000,China)

机构地区：[1]衡阳师范学院南岳学院生命科学系,湖南衡阳421000 [2]衡阳师范学院生命科学学院,湖南衡阳421000 [3]南岳山区生物资源保护与利用湖南省重点实验室,湖南衡阳421000

出　　处：《现代食品》2024年第11期81-85,91,共6页Modern Food

基　　金：2021年国家大学生创新创业训练计划项目(202112659001)。

摘　　要：采用凯氏定氮法检测杏鲍菇中蛋白质含量,并通过单因素和响应面试验对消化时间、消化温度及催化剂用量等条件进行了优化。结果显示,凯氏定氮法检测杏鲍菇中蛋白质含量的最优条件参数为消化时间1.85 h,消化温度438℃,催化剂浓硫酸用量14.8 mL,该条件下测定的杏鲍菇中蛋白质含量最高,为20.71 g/100 g。与国标法比较,本研究中优化后的方法提高了蛋白质含量的检测效率,减少了催化剂浓硫酸的用量,缩短了消化时间。The Kjeldahl nitrogen determination method was used to detect the protein content of Pleurotus eryngii,and the digestion time,digestion temperature and catalyst dosage were optimized by single factor and response surface tests.The results showed that the optimal parameters for the detection of protein content in Pleurotus eryngii by Kjeldahl nitrogen determination method were the digestion time of 1.85 h,the digestion temperature of 438℃,and the amount of concentrated sulfuric acid in the catalyst was 14.8 mL,and the protein content of Pleurotus eryngii was the highest(20.71 g/100 g)under these conditions.Compared with the national standard method,the optimized method in this study improved the detection efficiency of protein content,reduced the amount of concentrated sulfuric acid in the catalyst,and shortened the digestion time.

关 键 词：蛋白质 响应面分析法 凯氏定氮法 杏鲍菇 

分 类 号：TQ936.2[化学工程] TS201.4[轻工技术与工程—食品科学]