三个护肝产品对醉酒模型大鼠解酒护肝作用功效研究  

Study on the Effect of Three Liver-Protecting Products on the Anti-alcohol and Liver-Protection in Drunken Model Rat

作　　者：严家荣[1,2] 潘晓慧 黎耀俊 郭起岳[1,2] 黄远英 YAN Jiarong;PAN Xiaohui;LI Yaojun;GUO Qiyue;HUANG Yuanying(Guangdong Medical Laboratory Animal Center,Foshan 528248;Guangdong Provincial People’s Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510080;By-Health Co.,Ltd.,Guangzhou 510663)

机构地区：[1]广东省医学实验动物中心,佛山528248 [2]广东省人民医院,广东省医学科学院,广州510080 [3]汤臣倍健股份有限公司,广州510663

出　　处：《食品工业》2024年第6期80-85,共6页The Food Industry

基　　金：广东省中医药局科研项目(项目编号:20211058)。

摘　　要：研究三个护肝产品对醉酒模型大鼠的解酒护肝作用。大鼠随机分为正常对照组,模型对照组,阳性对照组,样品1低、高剂量组,样品2低、高剂量组,样品3低、高剂量组。正常对照组、模型对照组给予去离子水灌胃,阳性对照组给予海王牌金樽片混悬液,样品1,2和3低、高剂量组分别灌胃相应受试物,连续灌胃14 d,并于末次给予受试物1 h后,除正常对照组外,其余各组采用红星二锅头稀释液(浓度3∶1)制备大鼠醉酒模型。通过翻正反射试验,观察每组动物醉酒时间、醉酒数、醒酒时间、2 h内醉酒率;检测血液乙醇和乙醛体积分数;检测血清及肝脏中乙醇脱氢酶(ADH)和乙醛脱氢酶(ALDH)活性;取肝脏进行苏木素-伊红(HE)染色。结果显示,与模型对照组比较,样品1,2和3均能延长醉酒时间(P<0.01),缩短醒酒时间(P<0.05或P<0.01),降低血乙醇体积分数(P<0.05或P<0.01),样品1和2能显著提高血液ADH和ALDH活性(P<0.01),样品2和3对肝脏ADH和ALDH活性有显著提高作用(P<0.05或P<0.01),三个护肝产品作用效果未发现剂量依赖性。试验结果表明,三个护肝产品对乙醇所致的大鼠醉酒模型有较好的解酒、护肝作用,为后续解酒护肝功能性产品的研发提供科学依据。Study the anti-alcohol and liver-protecting effects of three liver-protecting products on drunk model rats.Rats were randomly divided into normal control group,model control group,positive control group,sample 1 low and high dose groups,sample 2 low and high dose groups,sample 3 low and high dose groups.The normal control group and the model control group were administered with deionized water,and the positive control group was administered with Haiwang Jinzun tablet suspension.The test substance was continuously administered orally for 14 days,and 1 hour after the last administration of the test substance,except for the normal control group,the rest of the groups used Red Star Erguotou diluent(concentration 3∶1)to prepare the rat drunk model.Observe the drunk time,number of drunks,sobering time and drunk rate within 2 hours of animals in each group by righting reflex test;Detect the blood ethanol and acetaldehyde concentration;Detect the alcohol dehydrogenase(ADH)and acetaldehyde dehydrogenase in serum and liver(ALDH)activity;The liver was stained with hematoxylin-eosin(HE)to observe the pathological conditions of the liver tissue.The result showed that compared with the model control group,samples 1,2,and 3 could prolong the drunk time(P<0.01),shorten the sober time(P<0.05 or P<0.01),and reduce the blood alcohol concentration(P<0.05 or P<0.01).Samples 1 and 2 could significantly increase blood ADH and ALDH activity(P<0.01).Samples 2 and 3 could significantly improve liver ADH and ALDH activity(P<0.05 or P<0.01).The effect of the three liver protection products no dose dependence was found.The test showed that the three liver-protecting products had a good anti-alcohol and liver-protecting effect on the rat model of alcohol-induced intoxication,which provided a scientific basis for the subsequent research and development of anti-alcohol and liver-protecting functional products.

关 键 词：护肝产品 大鼠醉酒模型 翻正反射试验 乙醇脱氢酶 乙醛脱氢酶 

分 类 号：TS201.4[轻工技术与工程—食品科学]