UV-B irradiation enhances the accumulation of beneficial glucosinolates induced by melatonin in Chinese kale sprout  

作　　者：Mengyu Wang Jiansheng Wang Yuhe Yang Zhiqing Li Ghazala Mustafa Yubo Li Lihong Liu Zuofa Zhang Qiaomei Wang 

机构地区：[1]Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang 310021,China [2]Key Laboratory of Horticultural Plant Growth,Development and Quality Improvement,Ministry of Agriculture,Department of Horticulture,Zhejiang University,Hangzhou,Zhejiang 310058,China [3]Department of Plant Sciences,Quaid-i-Azam University,Islamabad 45320,Pakistan

出　　处：《Horticultural Plant Journal》2024年第4期995-1006,共12页园艺学报（英文版）

基　　金：supported by grants from the National Science Foundation of China (Grant Nos.32202466,32172593,and32272746)。

摘　　要：Cruciferous sprout is a new form of vegetable product rich in bioactive compounds,especially glucosinolates.Previous studies have focused on increasing the accumulation of glucosinolates in cruciferous sprouts by applying different chemical regulators,with a particular focus on their contribution to nutritional quality and health benefits.Nevertheless,the effects of melatonin and UV-B irradiation on glucosinolate biosynthesis remain unclear.In this study,it was found that changes in melatonin concentrations significantly affected the contents of individual as well as total aliphatic and indolic glucosinolates.The 5μmol·L^(-1)melatonin was decided as the optimum concentration that could increase the content of beneficial glucosinolates including glucoraphanin and 4-methoxy glucobrassicin in Chinese kale sprouts.Notably,the enhancement of glucosinolate accumulation by melatonin treatment could be further amplified by UV-B irradiation.Furthermore,our results showed that R2R3-MYB transcription factor BoaMYB28 and BoaMYB51,which are central regulators of aliphatic and indolic glucosinolate biosynthesis respectively,were both involved in the regulation of glucosinolate biosynthesis by melatonin and UV-B irradiation.Additionally,the expression of glucosinolate biosynthetic genes,including BoaCYP79F1,BoaCYP83A1,BoaSUR1,BoaUGT74B1,BoaCYP79B2,BoaCYP79B3,and BoaCYP83B1 participated in the formation of core structures and BoaFMOGS-OX5,BoaAOP2,BoaCYP81F2,and BoaIGMT1 involved in the sidechain modification of aliphatic and indolic glucosinolate,was regulated by melatonin or UV-B irradiation.Taken together,these findings provide a potential strategy for improving the nutritional quality and resistance of Chinese kale sprouts.

关 键 词：MELATONIN Chinese kale SPROUTS UV-B GLUCOSINOLATES Nutritional quality Second metabolism 

分 类 号：S635.9[农业科学—蔬菜学]