Dual-functional microneedle with programmatic regulation of macrophage for autoimmune psoriasis treatment  

作　　者：Ze Qiang Zhao Bo Zhi Chen Jia Li Gan Yun Hao Feng Ling Liang Lingyun Yu Zi Yi Wang Samin Abbaszadeh Mohammad-Ali Shahbazi Ruixing Yu Xin Dong Guo 

机构地区：[1]State Key Laboratory of Organic-Inorganic Composites,Beijing University of Chemical Technology,Beijing 100029,China [2]Beijing Laboratory of Biomedical Materials,College of Materials Science and Engineering,Beijing University of Chemical Technology,Beijing 100029,China [3]China-Japan Friendship Hospital,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100029,China [4]Department of Dermatology,China-Japan Friendship Hospital,Beijing 100029,China [5]Department of Pharmaceutical Biomaterials,School of Pharmacy,Zanjan University of Medical Sciences,Zanjan 45139-56184,Iran [6]Department of Biomaterials and Biomedical Technology,University Medical Center Groningen,University of Groningen,Antonius Deusinglaan 1,9713 AV Groningen,The Netherlands [7]W.J.Kolff Institute for Biomedical Engineering and Materials Science,University of Groningen,Antonius Deusinglaan 1,9713 AV Groningen,The Netherlands

出　　处：《Nano Research》2024年第8期7436-7448,共13页纳米研究（英文版）

基　　金：supported by National Natural Science Foundation of China(Nos.52161145410,52373126,and 52303173);the National Natural Science Foundation of China-Iran National Science Foundation joint grant(No.4001987);Beijing University of Chemical Technology-China-Japan Friendship Hospital Biomedical Translational Engineering Research Center Key Projects(No.XK2023-20);the longterm subsidy mechanism from the Ministry of Finance and the Ministry of Education of People's Republic of China.M.-A.S.acknowledges the financial support of the incentive and startersbeurs funds from the University of Groningen,Groningen,The Netherlands.

摘　　要：Modulating the immune microenvironment to establish sustained positive feedback within immune pathways represents a promising avenue for the treatment of autoimmunity.However,the precise and efficient delivery of therapeutic systems to the subcutaneous basal layer to modulate immune disorders is a major challenge in the treatment of autoimmune psoriasis.In this project,we introduce a dual-functional microneedle(DF-MN)designed to combine MNs with multiple release kinetics and immunotherapy,the programmed treatment is achieved through segmented design of the MN structure,realizing the unification of rapid and long-lasting treatment of autoimmune psoriasis.In vivo imaging results showed that GelMA@M-CSF showed fluorescent signals after 5 days of delivery to subcutaneous tissues,whereas HA@IL-13 showed minimal fluorescent signals after 2 days.The multistage release behavior of MNs and the diffusion mechanism of drugs were explained at the molecular level,in combination with coarse-grained molecular dynamics.Additionally,DF-MN can successfully induce macrophage reprogramming in vitro and ameliorate overall symptoms in a psoriasis mice model,suggesting that it has the potential to be an effective strategy for the treatment of psoriasis and portends to be a transformative platform for the treatment of other autoimmune diseases.

关 键 词：MICRONEEDLE PSORIASIS M2 macrophage IMMUNOREGULATION 

分 类 号：R758.63[医药卫生—皮肤病学与性病学]