A gold-based multiplex immunochromatographic sensor for detecting paclitaxel  

作　　者：Xiaoqian Jiang Aihua Qu Xinxin Xu Hua Kuang Liqiang Liu Chuanlai Xu 

机构地区：[1]State Key Laboratory of Food Science and Technology,Jiangnan University,Wuxi 214122,China [2]International Joint Research Laboratory for Biointerface and Biodetection,and School of Food Science and Technology,Jiangnan University,Wuxi 214122,China

出　　处：《Nano Research》2024年第7期6420-6429,共10页纳米研究（英文版）

基　　金：financially supported by the National Natural Science Foundation of China(Nos.22236002 and 21925402);this work is also financially supported by National Key R&D Program(No.2021YFA1200300).

摘　　要：Paclitaxel(PTX),methotrexate(MTX),and 5-fluorouracil(5-FU)are commonly-used small molecule anti-tumor drugs for breast cancer.Unfortunately,drug resistance occurs with long-term treatment or excessive use,and the high concentrations of PTX,MTX,and 5-FU in patients may lead to side effects with dose-limiting toxicities,such as myelosuppression,hepatotoxicity,and peripheral neuropathy.Therefore,concentration monitoring of PTX,MTX,and 5-FU in clinical treatment is very important.We prepared highly sensitive and specific monoclonal antibodies using several haptens,and established a multiple paper-based sensor utilizing optical signals of gold nanoparticles,which simultaneously detected PTX,MTX,and 5-FU in human plasma or urine with 10 min.A portable scanner for quantitative detection in plasma and urine samples was used,and the calculated limit of detection(cLOD)values were 0.002 and 0.142μg/mL for PTX,0.187 and 0.976 ng/mL for MTX,and 0.057 and 0.128μg/mL for 5-FU,respectively.In the recovery test,the recovery rates and the coefficient of variation were 85.84%–108.81%and 1.23%–8.80%,respectively,indicating the reliability and accuracy of the multiple gold immunochromatographic strip(GIS).In addition,for the determination of collected clinical plasma samples,the correlation between the test data of the multiple GIS and liquid chromatography–tandem mass spectrometry(LC–MS/MS)was good.Therefore,the developed multiple GIS could be applied to the rapid detection of PTX,MTX,and 5-FU in different clinical samples.

关 键 词：PACLITAXEL METHOTREXATE 5-FLUOROURACIL immunochromatographic assay PLASMA URINE 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]