杜仲协同拟茎点霉XP-8合成木脂素类的条件优化及其功能活性  

Functional Activity and Optimization of Synthesis Factors of Lignans from Phomopsis sp.XP-8 Assisted Eucommia ulmoides Oliv.

作　　者：朱静[1,2] 陈晖 陈亚蓝 师俊玲[2] ZHU Jing;CHEN Hui;CHEN Yalan;SHI Junling(Food Science and Engineering College,Xinyang Agriculture and Forestry University,Xinyang 464000,China;School of Life Science,Northwestern Polytechnical University,Xi'an 710072,China)

机构地区：[1]信阳农林学院食品科学与工程学院,河南信阳464000 [2]西北工业大学生命学院,陕西西安710072

出　　处：《食品工业科技》2024年第15期360-368,共9页Science and Technology of Food Industry

基　　金：河南省重点研发与推广专项(科技攻关)项目(212102110314,242102110090);河南省青年科学基金项目(212300410228);信阳农林学院高水平科研孵化器建设基金项目(FCL202014);河南省高等学校重点科研项目(24B550017)。

摘　　要：对杜仲协同拟茎点霉XP-8生物合成松脂醇(Pinoresinol,Pin)、松脂醇单葡萄糖苷(Pinoresinol-4-O-β-Dglucopyranoside,PMG)和松脂醇二葡萄糖苷(Pinoresinol diglucoside,PDG)的条件进行优化,研究培养方式、杜仲添加量和静息处理时间对Pin、PMG和PDG产量的影响,并对相关产物的抗氧化性和抑癌性进行研究。结果表明,3%杜仲PDB培养基培养菌体并静息处理5 d时Pin、PMG和PDG产量较高。该条件下代谢产物液对DPPH和ABTS+自由基的清除能力显著高于杜仲空白培养基,说明发酵过程中产生了增强抗氧化功能的物质。其还能抑制肝癌细胞HepG-2的增殖,通过纯品验证活性说明,Pin是其中主要抑制癌细胞的活性成分,代谢产物液抑制肝癌细胞的作用是一种协同效应。Optimization of process conditions for biosynthesis of pinoresinol(Pin),pinoresinol-4-O-β-D-glucopyranoside(PMG)and pinoresinol diglucoside(PDG)by Phomopsis sp.XP-8 assisted Eucommia ulmoides Oliv.was performed.The effects of the cultured method,the addition of Eucommia ulmoides Oliv.and the time of resting on the production of Pin,PMG and PDG by Phomopsis sp.XP-8 assisted Eucommia ulmoides Oliv.were studied.The antioxidative,anticancer properties of the related products were also investigated.The results showed that the optimal yield of Pin,PMG and PDG was obtained at the condition with the PDB medium contained 3% Eucommia ulmoides Oliv.with resting treatment for 5 d.Under the optimal condition,the free radical scavenging ability for DPPH and ABTS+of metabolite liquid was significantly better than that of the blank culture medium.This indicated that the antioxidative substances were generated during the fermentation process.The hepatocellular carcinoma HepG-2 proliferation was suppressed by the extract solution.The pure product verifications of activity of the standard samples(Pin,PMG and PDG)were performed.It indicated that Pin was identified as the main active ingredient that suppressed HepG-2.The suppressed effect of the metabolite liquid on HepG-2 was a synergistic effect.

关 键 词：木脂素类物质 拟茎点霉XP-8 杜仲 抗氧化 抑癌 

分 类 号：TS201.1[轻工技术与工程—食品科学]