Black radish root extract alleviates sodium valproate-induced liver damage via inhibiting mitochondrial membrane potential collapse and oxidative stress in mice  

作　　者：Mohammad Hadi Zarei Sami Akbulut Maryam Zafari Elham Saghaei Zahra Lorigooini Hossein AminiKhoei Somaye Khosravi Elham Bijad 

机构地区：[1]Medical Plants Research Center,Basic Health Sciences Institute,Shahrekord University of Medical Sciences,Shahrekord,Iran [2]Department of Liver Transplant Institute and Biostatistics,Inonu University Faculty of Medicine,44280,Malatya,Turkey [3]Student Research Committee,Shahrekord University of Medical Sciences,Shahrekord,Iran [4]Physiology and Pharmacology Department,School of Medicine,Shahrekord University of Medical Sciences,Shahrekord,Iran [5]Department of Anatomical Sciences,School of Medicine,Shahrekord University of Medical Sciences,Shahrekord,Iran

出　　处：《Asian Pacific Journal of Tropical Biomedicine》2024年第7期298-306,共9页亚太热带生物医学杂志（英文版）

基　　金：supported by a research grant(No.6211)from Shahrekord University of Medical Sciences,Shahrekord,Iran.

摘　　要：Objective:To explore the effect of black radish(Raphanus sativus L.var niger)root extract on liver enzymes,oxidative stress,and histopathological alterations in mice with sodium valproate-induced hepatotoxicity.Methods:Thirty-two mice were divided into four groups:the control group received drinking water by gavage,the second group was administered with 100 mg/kg of sodium valproate,the third group received 300 mg/kg of black radish root extract,and the fourth group was given both sodium valproate(100 mg/kg)and black radish root extract(300 mg/kg).After 28 days,the mice were euthanized,and serum levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),and alkaline phosphatase(ALP),along with liver malondialdehyde(MDA),reactive oxygen species(ROS),mitochondrial parameters,tumor necrosis factor-alpha(TNF-α)gene expression,and histopathological changes were assessed.Results:Sodium valproate caused hepatic damage in mice,characterized by elevated serum levels of liver enzymes,increased MDA and ROS levels and TNF-αgene expression,as well as histopathological alterations.The black radish root extract significantly alleviated sodium valproate-caused hepatic injury by decreasing the serum levels of ALT and AST,MDA,ROS,TNF-αgene expression,as well as mitochondrial impairment,but did not have a significant effect on sodium valproate-induced histopathological changes.Conclusions:The black radish root extract demonstrates protective effects against sodium valproate-induced liver injury,possibly through mitigating oxidative stress,mitochondrial impairment,and inflammatory mediator expression.

关 键 词：Black radish Raphanus sativus Sodium valproate HEPATOTOXICITY Oxidative stress ANTI-INFLAMMATION 

分 类 号：R575[医药卫生—消化系统] R285.5[医药卫生—内科学]