A nitroreductase-responsive nanoprobe with homogeneous composition and high loading for preoperative non-invasive tumor imaging and intraoperative guidance  

作　　者：Jingqi Xin Shupeng Han Meichen Zheng Chenfeng Xu Zhongxi Huang Bin Wang Changmin Yu Feifei An Yu Ren 

机构地区：[1]Institute of Medical Engineering,Department of Biophysics,School of Basic Medical Science,Health Science Center,Xi'an Jiaotong University,Xi'an 710061,China [2]School of Public Health,Health Science Center,Xi'an Jiaotong University,Xi'an 710061,China [3]Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM),Nanjing Tech University,Nanjing 211816,China [4]Department of Breast Surgery,the First Affiliated Hospital of Xi'an Jiaotong University,Xi'an 710061,China

出　　处：《Chinese Chemical Letters》2024年第7期313-318,共6页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China(Nos.51903201,82172798);the Natural Science Foundation of Shaanxi Province(No.2023-YBSF-270);the Open Program of NHC Key Laboratory of Nuclear Medicine and Jiangsu Key Laboratory of Molecular Nuclear Medicine(No.KF202203);Horizontal Project of the First Affiliated Hospital of Xi'an Jiaotong University(No.202304174)。

摘　　要：Tumor microenvironment(TME)-activatable probes have been proven to effectively increase signal-tobackground ratios(SBRs)and improve the success rate of complete tumor resection.However,many fluorescence probes have to be loaded into a nanocarrier for tumor targeted delivery,which consequently encounters poor drug loading,heterogeneous composition and non-encapsulated drug aggregates occurred during nanoformulation fabrications.Herein,a nitroreductase(NTR)-activated“OFF-ON”near-infrared fluorescence nanoprobe,named Nano Bodipy,was synthesized by the spontaneous self-assembling of NTRresponsive dye-polyethylene glycol(PEG)amphiphilic polymer in water.The NTR-responsive dye acted as the hydrophobic segment in the amphiphilic polymer,yielding a homogeneous composition and a high loading of 12.2 wt%(according to calculation)in the synthesized Nano Bodipy.The synthesized Nano Bodipy can efficiently accumulate in tumors via the enhanced permeability and retention(EPR)effect,enabling non-invasive tumor-targeted fluorescence imaging and guiding complete tumor resection.Once the synthesized Nano Bodipy entered the tumor cells,they dissociated and were activated by overexpressed NTR.With the real-time fluorescence guide of Nano Bodipy,complete tumor resection surgery was performed successfully.

关 键 词：NITROREDUCTASE Tumor diagnosis Intraoperative guidance Self-assembly NANOPROBE 

分 类 号：R73-3[医药卫生—肿瘤] O657.3[医药卫生—临床医学]