Extracellular vesicles-coupled miRNAs from oviduct and uterus modulate signaling pathways related to lipid metabolism and bovine early embryo development  

作　　者：Rosane Mazzarella Karina Canon‑Beltran Yulia N.Cajas Meriem Hamdi Encina M.Gonzalez Juliano C.da Silveira Claudia L.V.Leal D.Rizos 

机构地区：[1]Department of Animal Reproduction,INIA-CSIC,Madrid,Spain [2]Department of Biochemistry and Molecular Biology,Veterinary Faculty,Complutense University of Madrid(UCM),Madrid,Spain [3]Department Agrarian Production,Technical University of Madrid,UPM,Madrid,Spain [4]Departamento de Ciencias Biologicas,Universidad Tecnica Particular de Loja,UTPL,Loja,Ecuador [5]Department of Anatomy and Embryology,FV-UCM,Madrid,Spain [6]Department of Veterinary Medicine,FZEA-USP,Pirassununga,Brazil

出　　处：《Journal of Animal Science and Biotechnology》2024年第4期1528-1549,共22页畜牧与生物技术杂志（英文版）

基　　金：PID2019-111641RB-I00 funded by MCIN/AEI/10.13039/501100011033/to DR and PRE2020-094452 to RM;FAPESP;#2017/20339–3 and CNPq-Brazil (304276/2018–9) to CLVL and FAPESP#2014/22887–0 and 2015/21829–9 to JCS;funded by S?o Paulo Research Foundation,Brazil;supported by a Maria Zambrano contract,and YNC for a Margarita Salas contract,both funded by the European Union–Next Generation EU program。

摘　　要：Background Extracellular vesicles(EVs) present in oviductal(OF) and uterine fluid(UF) have been shown to enhance bovine embryo quality during in vitro culture by reducing lipid contents and modulating lipid metabolism-related genes(LMGs), while also influencing cell proliferation, suggesting their involvement on the regulation of different biological pathways. The regulation of signaling pathways related to cell differentiation, proliferation, and metabo-lism is crucial for early embryo development and can determine the success or failure of the pregnancy. Bioactive molecules within EVs in maternal reproductive fluids, such as micro RNAs(miRNAs), may contribute to this regulatory process as they modulate gene expression through post-transcriptional mechanisms.Results This study evaluated miRNA cargo in OF-EVs from the early luteal phase and UF-EVs from the mid-luteal phase, coinciding with embryo transit within oviduct and uterus in vivo, and its possible influence on LMGs and sign-aling pathways crucial for early embryo development. A total of 333 miRNAs were detected, with 11 exclusive to OF, 59 to UF, and 263 were common between both groups. From the 20 differentially expressed miRNAs, 19 up-regulated in UF-EVs(bta-miR-134, bta-miR-151-3p, bta-miR-155, bta-miR-188, bta-miR-181b, bta-miR-181d, bta-miR-224, bta-miR-23b-3p, bta-miR-24-3p, bta-miR-27a-3p, bta-miR-29a, bta-miR-324, bta-miR-326, bta-miR-345-3p, bta-miR-410, bta-miR-652, bta-miR-677, bta-miR-873 and bta-miR-708) and one(bta-miR-148b) in OF-EVs. These miRNAs were predicted to modulate several pathways such as Wnt, Hippo, MAPK, and lipid metabolism and degradation. Differ-ences in miRNAs found in OF-EVs from the early luteal phase and UF-EVs from mid-luteal phase may reflect differ-ent environments to meet the changing needs of the embryo. Additionally, miRNAs may be involved, particularly in the uterus, in the regulation of embryo lipid metabolism, immune system, and implantation.Conclusions Our study suggests that miRNAs within OF- and UF-EVs could m

关 键 词：Embryo-maternal interaction EPIGENETIC Exosomes MAMMALIAN PREIMPLANTATION Reproductive fluids 

分 类 号：S823[农业科学—畜牧学]