纯种发酵淡豆豉的抗抑郁作用研究  

Antidepressant effect of pure fermented Sojae Semen Praeparatum

作　　者：冯驰[1] 马书伟 周立分[1] 熊京京 李翠英 戴家齐 谢小琴[1] 龙凯[1] 谢小梅[1] FENG Chi;MA Shuwei;ZHOU Lifen;XIONG Jingjing;LI Cuiying;DAI Jiaqi;XIE Xiaoqin;LONG Kai;XIE Xiaomei(Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学,江西南昌330004

出　　处：《药物评价研究》2024年第6期1224-1231,共8页Drug Evaluation Research

基　　金：国家自然科学基金资助项目(82060709,82360772);江西省教育厅科学技术研究项目(GJJ180655)。

摘　　要：目的初步研究纯种发酵淡豆豉的抗抑郁作用及其活性成分。方法制备自然发酵淡豆豉和枯草芽孢杆菌纯种发酵淡豆豉,采用显色鉴别、薄层鉴别、异黄酮和γ-氨基丁酸(GABA)的含量测定对纯种发酵淡豆豉进行质量评价;昆明小鼠按体质量随机分为8组,每组10只,雌雄各半,分别为对照组、模型组、盐酸氟西汀(阳性对照,0.01 g·kg^(-1))组、γ-氨基丁酸(GABA,0.01445 g·kg^(-1))组、异黄酮(0.00305 g·kg^(-1))组、自然发酵淡豆豉醇提水洗脱物(水洗脱物,1.82g·kg^(-1))组、自然发酵淡豆豉总提物(自然发酵,1.82 g·kg^(-1))组、纯种发酵淡豆豉总提物(纯种发酵,1.82 g·kg^(-1))组,除对照组外,建立慢性温和不可预知应激(CUMS)小鼠抑郁模型,边造模边给药,每日应激前1 h ig给药,连续给药28 d。实验第0、7、14、21、28天分别测定各组小鼠体质量,第28天给药1 h后,进行悬尾、强迫游泳、糖水偏好、敞箱实验各行为学指标检测;HE染色后观察海马CA1区病理学变化;超高效液相色谱-串联质谱法(UPLC-MS/MS)鉴定水洗脱物组、纯种发酵组的主要化学成分。结果纯种发酵淡豆豉与自然发酵淡豆豉有相同的显色反应、显色斑点和GABA含量。与模型组相比,各给药组在应激第14、21、28天时体质量增加明显(P<0.01),悬尾及强迫游泳不动时间显著降低(P<0.01);GABA组、异黄酮组、纯种发酵组、自然发酵水洗脱物组小鼠糖水偏好指数均显著增加(P<0.05、0.01);盐酸氟西汀组、水洗脱物组、GABA组、纯种发酵组小鼠敞箱实验跨格数和直立数均显著增加(P<0.05、0.01);各给药组海马CA1区细胞排列紧密有序,边缘圆润,细胞间隙正常。纯种发酵组与自然发酵总提物组、水洗脱物组小鼠的各项行为学指标均无显著差异。自然发酵醇提水洗脱物组、纯种发酵总提物组都含有大豆苷元、染料木素、GABA和各种氨基酸。结论纯种发酵淡豆豉具有�Objective A preliminary study on the antidepressant effect of pure-bred fermented Sojae Semen Praeparatum(PF-SSP)and its active components.Methods According to the established process in our laboratory,natural fermented Sojae Semen Praeparatum(NF-SSP)and PF-SSP by Bacillus subtilis were prepared.The quality evaluation of PF-SSP was carried out by colorimetric identification,thin-layer identification,and content determination of flavonoid and-aminobutyric acid(GABA).Divided Kunming mice into eight groups randomly by body weight,with 10 mice in each group,half male and half female.The groups were as follows:control group,model group,fluoxetine(positive control,0.01 g·kg^(-1))group,GABA(0.01445 g·kg^(-1))group,flavonoid(0.00305 g·kg^(-1))group,NF-SSP ethanol extract washout material(water washout material,1.82 g·kg^(-1))group,NF-SSP total extract(NF-SSP,1.82 g·kg^(-1))group,PF-SSP total extract group(PF-SSP,1.82 g·kg^(-1)).Except for the control group,an experimental model of chronic mild unpredictable stress(CUMS)in mice was established,and the mice were given drugs while being subjected to the model.Drugs were administered ig one hour before the stress every day for 28 consecutive days.Body weight was measured in mice at 0,7,14,21,and 28 days.The behavioral indicators of the hanging tail,forced swimming,sucrose preference,and open field test were detected 1 h after giving drugs on day 28.Hematoxylin and eosin(HE)staining was performed to observe pathological changes in the hippocampal CA1 area.UPLC-MS/MS was used to identify the main chemical components of the aqueous extract and pure strain fermentation products.Results PF-SSP and NF-SSP had the same color reaction,color spots and GABA content.Compared with model group,the body weight of each treatment group increased significantly at 14,21,and 28 days after stress(P<0.01),and the immobility time in the tail suspension and forced swimming test were significantly reduced(P<0.01);the sucrose preference index of GABA group,isoflavone group,PF-SSP group,and wat

关 键 词：纯种发酵 淡豆豉 抗抑郁 异黄酮 Γ-氨基丁酸 

分 类 号：R285.5[医药卫生—中药学]