香芹酚对恶臭假单胞菌的抑制作用  

Inhibitory Effect of Carvacrol on Pseudomonas putina

作　　者：李佳玟 吴轩 潘雅洁 肖龙泉[1,2] 刘达玉 王新惠[1] 徐纲[2] 周闯 LI Jiawen;WU Xuan;PAN Yajie;XIAO Longquan;LIU Dayu;WANG Xinhui;XU Gang;ZHOU Chuang(School of Food and Biological Engineering,Chengdu University,Chengdu 610106,China;Yibin E-Tiange Foods Co.Ltd.,Yibin 644100,China)

机构地区：[1]成都大学食品与生物工程学院,四川成都610106 [2]宜宾市娥天歌食品有限公司,四川宜宾644100

出　　处：《肉类研究》2024年第6期71-75,共5页Meat Research

基　　金：四川省科技计划项目(2023YFN00140);宜宾市科技计划项目(2022NY021);四川省肉羊创新团队建设项目(SCCXTD-2024-14);达州市科技计划项目(22DWHZ0005)。

摘　　要：以恶臭假单胞菌(Pseudomonas putida)为对象,通过测定恶臭假单胞菌生长曲线、群集性、泳动性、核酸泄漏情况及产胞外蛋白酶和生物被膜能力等指标,考察不同体积分数香芹酚对恶臭假单胞菌的抑菌效果。结果表明:当香芹酚体积分数≥0.015%时,对恶臭假单胞菌的生长、运动能力、产胞外蛋白酶和生物被膜能力具有极显著抑制作用(P<0.01);当香芹酚体积分数为0.025%时,恶臭假单胞菌所产生物被膜含量比空白组极显著减少86.35%(P<0.01)。香芹酚可通过抑制生长、产生物被膜能力及运动能力破环细胞膜通透性,导致大量细胞内容物泄漏,从而对恶臭假单胞菌产生抑菌作用。The inhibitory effect of different volume fractions(V/V) of carvacrol on Pseudomonas putida was investigated through the determination of the growth curve,swarming motility,swimming motility,nucleic acid leakage,the ability to produce extracellular protease and biofilm of P.putida.The results showed that carvacrol at a volume fraction equal to or greater than 0.015% had a significant inhibitory effect on the growth,motility,extracellular protease production and biofilm formation of P.putida(P < 0.01).When the volume fraction of carvacrol was 0.025%,the biofilm production of P.putida was reduced by 86.35% compared with the blank group(P < 0.01).Carvacrol can exert an inhibitory effect on P.putida by inhibiting its growth,biofilm-producing ability and motility,and destroying the permeability of the cell membrane,and thereby resulting in the leakage of a large amount of cellular contents.

关 键 词：恶臭假单胞菌 香芹酚 鲜肉保鲜 泳动性 群集性 

分 类 号：TS251.1[轻工技术与工程—农产品加工及贮藏工程]