小麦水解蛋白肽段组成及结构序列分析  

Composition and Structural Sequence Analysis of Hydrolyzed Wheat Protein Peptides

作　　者：方磊 夏羽菡[2] 任杰 崔硕 吴晗硕 卢知浩 马永庆 张新雪 刘文颖 FANG Lei;XIA Yuhan;REN Jie;CUI Shuo;WU Hanshuo;LU Zhihao;MA Yongqing;ZHANG Xinxue;LIU Wenying(Beijing Engineering Research Center of Protein and Functional Peptides,China National Research Institute of Food and Fermentation Industries Co.Ltd.,Beijing 100015,China;General Hospital of Ningxia Medical University,Yinchuan 750004,China;School of Biomedicine,Beijing City University,Beijing 100083,China)

机构地区：[1]中国食品发酵工业研究院有限公司北京市蛋白功能肽工程技术研究中心,北京100015 [2]宁夏医科大学总医院,宁夏银川750004 [3]北京城市学院生物医药学部,北京100083

出　　处：《食品与生物技术学报》2024年第6期104-111,共8页Journal of Food Science and Biotechnology

基　　金：宁夏回族自治区重点研发计划项目(2021BEG02027)。

摘　　要：对小麦水解蛋白的基础理化成分、相对分子质量分布和氨基酸组成进行分析,并利用LC-MS/MS法鉴定小麦水解蛋白肽段组成及其序列。结果显示,小麦水解蛋白的总蛋白质质量分数为(80.53±8.06)%,相对分子质量主要集中在小于2000的范围,必需氨基酸的质量分数为52.4%。根据Q3扫描、PIS扫描以及MRM定量共鉴定出8条肽段:亮氨酰谷氨酰胺二肽(leucylglutamine,LQ)、缬氨酰谷氨酰胺二肽(valanyl-glutamine,VQ)、异亮氨酰异亮氨酸二肽(isoleucylisoleucine,Ⅱ)、亮氨酰亮氨酸二肽(leucyl-leucine,LL)、丙氨酰异亮氨酸二肽(alanyl-isoleucine,AI)、缬氨酰亮氨酸二肽(valanyl-leucine,VL)、亮氨酰甲硫氨酸二肽(leucyl-methionine,LM)和异亮氨酰甲硫氨酸二肽(isoleucyl-methionine,IM),质量分数分别为385.0、32.5、30.0、122.8、8.9、5.9、134.9、147.0μg/g。它们可能在体内发挥功能活性,成为小麦水解蛋白应用于营养保健食品的物质基础。Hydrolyzed wheat protein was analyzed by basic physicochemical components,relative molecular mass distribution,and amino acid composition.The peptide composition and sequence were identified by LC-MS/MS.The results showed that the total protein mass fraction of hydrolyzed wheat protein was(80.53±8.06)%,with the relative molecular mass mainly below 2000 and a mass fraction of essential amino acids approximately 52.4%.Eight peptides were identified by Q3 scanning,PIS scanning,and MRM quantification,including leucyl-glutamine(LQ),valanyl-glutamine(VQ),isoleucyl-isoleucine(Ⅱ),leucyl-leucine(LL),alanyl-isoleucine(AI),valanyl-leucine(VL),leucyl-methionine(LM)and isoleucyl-methionine(IM),with mass fractions of 385.0,32.5,30.0,122.8,8.9,5.9,134.9,147.0μg/g,respectively.These peptides may exhibit functional activities in vivo and serve as the material foundation for the application of hydrolyzed wheat protein in nutritional health food.

关 键 词：小麦 水解蛋白 相对分子质量 氨基酸 肽段鉴定 质谱 

分 类 号：TS253.9[轻工技术与工程—农产品加工及贮藏工程]