硅烷衍生化GC-MS检测香精中糖类物质成分  

Determination of saccharides in essence by silane derivatization combined with GC-MS

作　　者：邹琳[1] 王云龙 刘茜[1] 李坚[1] 杨龙彦[1] 宁振兴[1] ZOU Lin;WANG Yun-long;LIU Qian;LI Jian;YANG Long-yan;NING Zhen-xing(Guangxi Tobacco Industry Co.,Ltd.,Technology Center,Nanning 530001,Guangxi,China;Henan Provincial Flavor and Fragrance Engineering Technology Research Center,College of Tobacco,Henan Agricultural University,Zhengzhou 450002,Henan,China)

机构地区：[1]广西中烟工业有限责任公司技术中心,广西南宁530001 [2]河南农业大学烟草学院,河南省香精香料与调香工程技术研究中心,河南郑州450002

出　　处：《粮食与油脂》2024年第8期122-126,131,共6页Cereals & Oils

基　　金：国家自然科学基金项目(21801064);广西中烟工业有限责任公司科技项目(2022450000340053)。

摘　　要：建立了基于硅烷衍生化气相色谱-质谱联用法快速检测香精中17种糖类物质的方法。结果表明:衍生化的最佳条件为以BSTFA为衍生化试剂、衍生化温度40℃、衍生化时间40 min,在此条件下共鉴定出17种糖类物质。17种糖类物质在一定质量浓度内具有良好的线性关系(R^(2)>0.99)。方法的检出限为0.027~0.077μg/mL,加标回收率为94.80%~110.03%,相对标准偏差为3.03%~8.06%。实际样品测定结果表明,建立的方法能够快速准确地检测香精中17种糖类物质成分,满足对香精中糖类物质的同时检测。A method based on silane derivatization gas chromatography-mass spectrometry was developed for the rapid detection of 17 kinds of saccharides in essence.The results showed that the optimal conditions for derivatization were using BSTFA as derivatization reagent,derivatization temperature 40℃ and derivatization time 40 min.Under these conditions,17 saccharides were identified.17 saccharides exhibited good linear relationship within certain mass concentration during detection(R^(2)>0.99).The detection limit of this method was 0.027-0.077μg/mL,the recovery rate was 94.80%-110.03%,and the relative standard deviation of 3.03%-8.06%.The actual sample determination results showed that the method could quickly and accurately detect 17 kinds of saccharides in essence,and could meet the simultaneous detection of saccharides in essence.

关 键 词：硅烷衍生化 气相色谱-质谱法(GC-MS) 检测 糖类物质 

分 类 号：TS207.3[轻工技术与工程—食品科学]