Rapamycin Potentiates the Antitumor Effect of 5-Fluorouracil in Colon Cancer by Enhancing Autophagy  

作　　者：Xiaoming Tian Youjun He Tao Hao 

机构地区：[1]Feicheng City People’s Hospital,Shandong,271000,China [2]Department of Colorectal Hernia Surgery,Binzhou Medical University Hospital,Shandong,256600,China

出　　处：《Journal of Clinical and Nursing Research》2024年第7期152-160,共9页临床护理研究（英文）

摘　　要：Objective:To investigate whether the mTOR inhibitor rapamycin can enhance the growth suppression effect of 5-fluorouracil(5-FU)on colon cancer cells.Methods:CCK8 assays were used to examine cell survival.Flow cytometry and Western blotting were employed to detect cell proliferation,apoptosis,and related markers.Results:The combination of rapamycin and 5-FU exhibited greater cytotoxicity in cells compared to rapamycin or 5-FU alone.Notably,cells in the G0/G1 phase increased while cells in the S phase decreased in the combination group,consistent with changes in the levels of Cyclin D1 and PCNA.Rapamycin enhanced 5-FU-induced apoptosis in vitro by inducing caspase-dependent apoptosis,which is Bax/Bcl-2 related.Conclusion:The combination of rapamycin and 5-FU showed a significant synergistic anticancer effect by enhancing autophagy.This study supports that the combination of rapamycin with 5-FU is an effective and feasible approach for colorectal cancer treatment.

关 键 词：Colorectal cancer Proliferation Apoptosis AUTOPHAGY RAPAMYCIN 

分 类 号：R73[医药卫生—肿瘤]