SPME-气相色谱串联质谱法检测食品中的环氧乙烷及其代谢物2-氯乙醇  

Determination of ethylene oxide and its metabolite 2-chloroethanol in food by SPME-gas chromatography tandem mass spectrometry

作　　者：肖桂英 池连学 王丹 郑振佳[3] 李帅 张禧庆 Xiao Guiying;Chi Lianxue;Wang Dan;Zheng Zhenjia;Li Shuai;Zhang Xiqing(Shandong Jienuo Testing Service Co.,Ltd.,Yantai 265231,China;Longda Foodstuff Group Co.,Ltd.,Yantai 265231,China;College of Food Science and Engineering,Shandong Agricultural University,Key Laboratory of Food Processing Technology and Quality Control,Shandong Provincial University,Taian 271018,China)

机构地区：[1]山东杰诺检测服务有限公司,烟台265231 [2]龙大食品集团有限公司,烟台265231 [3]山东农业大学食品科学与工程学院,山东省高校食品加工技术与质量控制重点实验室,泰安271018

出　　处：《分析仪器》2024年第4期31-35,共5页Analytical Instrumentation

摘　　要：建立了顶空固相微萃取(solid phase micro extraction,SPME)-气相色谱串联质谱(GC-MS/MS)法检测食品中的环氧乙烷及其代谢物2-氯乙醇的分析方法。样品采用SPME(萃取纤维头95umC-WR/PDMS)在线提取,使用毛细管色谱柱HP-VOC分离环氧乙烷和2氯乙醇及其他干扰组分,高效电子轰击电离源(HES)离子化,多反应监测(multiple reaction monitoring,MRM)扫描,外标法定量。环氧乙烷及2-氯乙醇在10~200μg/L线性范围内,线性关系良好(r>0.995),以环氧乙烷表示的环氧乙烷和2氯乙醇的总和方法定量限20μg/kg,在1倍、2倍和10倍定量限3个浓度添加水平下,水果、蔬菜、谷物及调味料食品中添加回收率在76.32%~102.05%之间,重复性精密度在1.66%~5.96%之间。该方法灵敏度高、准确度高、重现性好,适用于食品中环氧乙烷及其代谢物2-氯乙醇的检测。An analytical method for the determination of ethylene oxide and its metabolite 2-chloroethanol in food by gas chromatography tandem mass spectrometry was established.The sample was extracted online using SPME(extraction fiber head 95umC-WR/PDMS),separating ethylene oxide and 2-chloroethanol by capillary chromatography column HP-VOC,HES ionization,multiple reaction monitoring(MRM) scanning,quantitative analysis using external standard method.The calibration curves of ethylene oxide and 2-chloroethanol were linear in the range of 10-200μg/L with the correlation coefficients more than 0.995.The determination limit of Sum of ethylene oxide and 2-chloroethanol represented by ethylene oxide is 20μg/kg.Under the three concentration addition levels of 1LOQ、 2LOQ and 10LOQ,fruits,vegetables,grains,and adjusting auxiliary materials of food is 76.32%-102.05%,and the repeatability precision is 1.66%-5.96%.The method has high sensitivity,high accuracy and good reproducibility.It is suitable for the detection of ethylene oxide and 2-chloroethanol in food.

关 键 词：气相色谱-串联质谱仪 顶空固相微萃取 萃取纤维头 毛细管色谱柱 高效电子轰击电离源 食品 

分 类 号：TS207.5[轻工技术与工程—食品科学] O657.63[轻工技术与工程—食品科学与工程]