紫苏籽油对小鼠肠道微生物及抗氧化能力的影响  

Effects of Perilla seed oil on intestinal microorganisms andantioxidant capacity in mice

作　　者：陈雪梅 胡博 唐晓姝[1,2] 张白曦 CHEN Xuemei;HU Bo;TANG Xiaoshu;ZHANG Baixi(National Engineering Research Center for Function Food,Jiangnan University,Wuxi 214122,China;Food Science and Technology,Jiangnan University,Wuxi 214122,China)

机构地区：[1]江南大学国家功能食品工程技术研究中心,江苏无锡214122 [2]江南大学食品学院,江苏无锡214122

出　　处：《食品与发酵工业》2024年第16期271-277,共7页Food and Fermentation Industries

摘　　要：紫苏籽油含有丰富的α-亚麻酸、生育酚、植物甾醇以及多酚类等多种活性成分,通过研究紫苏籽油对肠道微生物及抗氧化能力的影响,为紫苏籽油的开发应用提供依据。将50只KM雄性小鼠随机分为空白组、模型组及紫苏籽油低(ZS-L,1.25 mL/kg BW)、中(ZS-M,2.5 mL/kg BW)、高(ZS-H,5 mL/kg BW)剂量组,干预30 d后,收集空白组以及紫苏籽油干预组粪便进行肠道菌群测序,通过微生物多样性、物种丰度及差异性变化,研究紫苏籽油对小鼠肠道微生物的影响。建立乙醇氧化损伤模型,测定生长性能指标以及血清中抗氧化酶超氧化歧化酶(super oxide dismutase, SOD)、丙二醛(malondialdehyde, MDA)、还原性谷胱甘肽(glutathione, GSH)和蛋白质羰基(protein carbonyl, PCO)的含量,研究紫苏籽油对小鼠抗氧化能力的影响。结果表明,与空白组相比,紫苏籽油组菌群种类增加且物种发生显著改变,提示紫苏油具有减肥、抗氧化应激作用。与乙醇氧化损伤模型组相比,低、中、高剂量组PCO、MDA含量显著降低,中、高剂量组SOD含量显著升高,高剂量组GSH含量显著升高、体重显著降低,心脏、肾脏体比显著升高。综上所述,紫苏籽油可调节小鼠肠道菌群,且具有较强的抗氧化能力和一定的减肥功效。Perilla seed oil contains rich and active ingredients such asα-linolenic acid,tocopherols,phytosterols,and polyphenols.Studying the effects of Perilla seed oil on the antioxidant activity and intestinal microorganisms can deliver valuable information for its development and application.Fifty KM male mice were randomly divided into 5 groups,including blank group,model group,and Perilla seed oil groups.The Perilla seed oil groups consisted of low dose group(1.25 mL/kg BW),medium dose group(2.5 mL/kg BW),and high dose group(5 mL/kg BW).The feces of the blank group and Perilla seed oil groups were collected for intestinal microflora sequencing after intervention for 30 days.The effects of Perilla seed oil on the intestinal microorganisms of mice were studied by detecting microbial diversity,species abundance,and differential changes in different mice groups.An ethanol oxidative damage model was established,and the effects of Perilla seed oil on the antioxidant capacity of mice were evaluated by measuring the growth performance indexes and the contents of superoxide dismutase(SOD),malondialdehyde(MDA),glutathione(GSH),and protein carbonyl(PCO).Compared with the blank group,the results showed that the amount of fecal microbiota of the Perilla seed oil mice group increased,and the species changed significantly,indicating that Perilla seed oil had effects of weight loss and antioxidant stress.Compared with the ethanol oxidative damage model group,the PCO and MDA were significantly reduced in Perilla seed oil mice group,the SOD was significantly increased in the medium and high dose Perilla seed oil mice groups,GSH was increased,the mice weight was significantly reduced,and the visceral-body ratio of heart and kidney were improved in high dose Perilla seed oil mice group.In conclusion,Perilla seed oil can regulate the intestinal flora of mice,improve anti-oxidation capacity,and slightly enhance the weight loss efficiency.

关 键 词：紫苏籽油 小鼠生长性能 乙醇氧化损伤 抗氧化能力 微生物多样性 

分 类 号：TS225.1[轻工技术与工程—粮食、油脂及植物蛋白工程] TS201.4[轻工技术与工程—食品科学与工程]