银耳酵素体外消化对抗氧化活性的影响  

Effects of Tremella fuciformis fermentation on antioxidant activity in vitro digestion

作　　者：孙嘉蔚 张恩泽 代瀚哲 单振广 李强 张平平 Sun Jiawei;Zhang Enze;Dai Hanzhe;Shan Zhenguang;Li Qiang;Zhang Pingping(College of Food Science and Bioengineering,Tianjin Agricultural University,Tianjin 300392,China;Chengde Dajinxing Food Co.,Ltd.,Luanping 068253,Hebei Province,China;Tianjin Qixin Food Group Co.Ltd.,Tianjin 301599,China)

机构地区：[1]天津农学院食品科学与生物工程学院,天津300392 [2]承德大金星食品有限公司,河北滦平068253 [3]天津齐心食品集团有限公司,天津301599

出　　处：《天津农学院学报》2024年第4期34-38,共5页Journal of Tianjin Agricultural University

基　　金：横向项目(TNHXKJ2023019)。

摘　　要：为研究体外消化对银耳酵素抗氧化活性的影响,以酵母菌、乳酸菌、酵母菌和乳酸菌混合菌种发酵的3种银耳酵素为消化对象,以·OH清除率、DPPH清除率、·O_(2)^(-)清除率和铁原子还原力为抗氧化性指标,分析它们在体外模拟消化过程中抗氧化能力的变化规律。3种银耳酵素在体外胃肠消化过程中,除了酵母发酵银耳酵素的DPPH清除能力在肠消化阶段有降低外,其余的抗氧化性均增强,且胃液比肠液更利于促进抗氧化能力的提升。3种银耳酵素经模拟胃肠消化后仍具有较高的抗氧化活性,其中混合菌种发酵银耳酵素的·OH和DPPH清除率最高,乳酸菌发酵银耳酵素的·O_(2)^(-)清除能力最强,二者的铁离子还原力相近,可为开发具有抗氧化活性的银耳酵素提供科学依据。To study the effects of in vitro digestion on antioxidant activity of Tremella fuciformis fermentation,three kinds of fermentation by saccharomyces,lactic acid bacteria,and mixed strains used as digestion objects,and·OH,DPPH,O_(2)^(-)clearance rate and iron atomic reducing power used as antioxidant indexes,the changes of their antioxidant capacity during simulated digestion in vitro were analyzed.In the process of in vitro gastrointestinal digestion,the antioxidant activity of the three Tremella fuciformis fermentation was significantly enhanced except that the DPPH scavenging ability of the saccharomyces fermented Tremella fuciformis fermentation was reduced in the intestinal digestion stage,and the gastric fluid was more conducive to promoting the antioxidant capacity than the intestinal fluid.The three Tremella fuciformis fermentation still showed high antioxidant activity after simulated digestion environment,among which the·OH and DPPH scavenging rates of the mixed strains were the highest,and the·O_(2)^(-)scavenging ability of the lactic acid bacteria was the strongest.The iron reducing power of the two kinds of fermentation was similar,which provides a scientific basis for the development of Tremella fuciformis fermentation with antioxidant activity.

关 键 词：银耳 酵素 体外消化 抗氧化活性 

分 类 号：TS219[轻工技术与工程—粮食、油脂及植物蛋白工程]