基于宏基因组测序对中国北方地区生乳中嗜冷菌的群落结构研究  

Study on the Community Structure of Psychrophilic Bacteria in Raw Milk from North Regions of China based on Metagenomic Sequencing

作　　者：许文君 郑楠[2,3] 王加启[2,3] 孟璐[2,3] XU Wenjun;ZHENG Nan;WANG Jiaqi;MENG Lu(College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China;Key Laboratory of Quality and Safety Control for Milk and Dairy Products of Ministry of Agriculture and Rural Affairs,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;Laboratory of Quality and Safety Risk Assessment for Dairy Products of Ministry of Agriculture and Rural Affairs(Beijing),Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]青岛农业大学食品科学与工程学院,青岛266109 [2]中国农业科学院北京畜牧兽医研究所农业农村部奶及奶制品质量安全控制重点实验室,北京100193 [3]中国农业科学院北京畜牧兽医研究所农业农村部奶产品质量安全风险评估实验室(北京),北京100193

出　　处：《畜牧兽医学报》2024年第8期3659-3668,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家重点研发计划(2022YFD1301003);农产品质量安全风险评估(GJFP20220304);国家奶牛产业技术体系(CARS36);中国农业科学院科技创新工程(ASTIP-IAS12)。

摘　　要：本研究旨在利用宏基因组测序探究中国北方地区生乳中嗜冷菌群落结构的多样性。在冬季从中国内蒙古地区和北京地区的14个牧场共采集14份生乳样品,分离纯化可培养嗜冷菌和提取生乳中微生物总DNA,并利用16S rDNA测序和宏基因组测序结合的方法对生乳中嗜冷菌进行分析。结果发现,北京地区和内蒙古地区生乳中嗜冷菌的群落结构无显著性差异。两个地区生乳中优势嗜冷菌属均是假单胞杆菌属、寡养单胞菌属和短波单胞杆菌属。全局和概述图谱、氨基酸代谢和碳水化合物代谢是最主要的功能代谢途径,且嗜冷菌中含有多种抗生素抗性基因。综上,本研究利用传统培养和宏基因组测序准确测定了生乳中嗜冷菌群落的物种和功能信息,有助于了解生乳中嗜冷菌的生命活动及为嗜冷菌风险评估提供基础信息,促进中国北京及内蒙古牧场开展防控。In this study,the aim The aim of this study was to investigate the community structure diversity of psychrophilic bacteria in raw milk in northern China by metagenomic sequencing.A total of 14 raw milk samples were collected from 14 pastures in Inner Mongolia and Beijing,China in winter.The culturable psychrophilic bacteria were isolated and purified and,total microbial DNA in raw milk was extracted as well.Moreover,the psychrophilic bacteria in raw milk were analyzed by 16S rDNA sequencing and metagenomic sequencing,respectively.The results showed that no significant difference was found in community structure of psychrophilic bacteria in raw milk between Beijing and Inner Mongolia.Pseudomonas,Stenotrophomonas and Brevundimonas were the dominant psychrophiles bacteria in raw milk from the two regions.The global and overview profiles,amino acid metabolism and carbohydrate metabolism were the most important functional metabolic pathways,and psychrophilic bacteria contained a variety of antibiotic resistance genes.In conclusion,this study used traditional culture and metagenomic sequencing to accurately determine the species and functional information of the psychrophilic community in raw milk,which is helpful to understand the life activities of psychrophilic bacteria in raw milk and provide basic information for risk assessment of psychrophilic bacteria.Furthermore,it lays down foundational knowledge pivotal for the effective prevention and management of pasture-related issues in Beijing and Inner Mongolia,China.

关 键 词：生乳 嗜冷菌 宏基因组测序 地区 结构 

分 类 号：TS252.1[轻工技术与工程—农产品加工及贮藏工程]