通过式固相萃取-高效液相色谱法测定糕点中15种抗氧化成分  

Determination of 15 kinds of antioxidant components in pastry by pass-through solid phase extraction and high performance liquid chromatography

作　　者：赵娟 单吉浩[2] 李智文 于福利 张利[4] 雷一越 赵彩会 薛妮妮 王璐 贺习文 ZHAO Juan;SHAN Ji-Hao;LI Zhi-Wen;YU Fu-Li;ZHANG Li;LEI Yi-Yue;ZHAO Cai-Hui;XUE Ni-Ni;WANG Lu;HE Xi-Wen(Shaanxi Qinyun Agricultural Products Inspection and Testing Co.,Ltd.,Weinan 714000,China;Institute of Food Science and Technology,Chinese Academy of Agricultural Science,Beijing 100193,China;Agricultural Inspection and Testing Center of Shaanxi Province,Xi’an 710000,China;Chongqing Animal Disease Prevention and Control Center,Chongqing 401120,China;Shaanxi Provincial Animal Husbandry Technology Extension Station,Xi’an 710016,China;Agricultural Technology Extension Center of Huayin City,Weinan City,Shaanxi Province,Weinan 714200,China)

机构地区：[1]陕西秦云农产品检验检测股份有限公司,渭南714000 [2]中国农业科学院农产品加工研究所,北京100193 [3]陕西省农业检验检测中心,西安710000 [4]重庆市动物疫病预防控制中心,重庆401120 [5]陕西省畜牧技术推广总站,西安710016 [6]陕西省渭南市华阴市农业技术推广中心,渭南714200

出　　处：《食品安全质量检测学报》2024年第14期195-202,共8页Journal of Food Safety and Quality

摘　　要：目的建立通过式固相萃取-高效液相色谱法同时测定糕点中7种天然抗氧化成分和8种合成抗氧化剂的定量分析方法。方法目标物采用1%乙酸乙腈溶液提取,用通过式亲水亲油平衡(hydrophilic and oleophilic balance,HLB)固相萃取柱净化,净化液浓缩处理后供高效液相色谱仪分析,15种目标物以0.5%甲酸溶液-甲醇为流动相梯度洗脱,C18色谱柱分离,二极管阵列检测器280 nm处测定。结果15种抗氧化成分在1~50μg/mL范围内有良好的线性关系,表没食子酸儿茶素的检出限(limit of detection,LOD)为2 mg/kg,定量限(limit of quantification,LOQ)为5 mg/kg,其余14种化合物的LOD为0.2 mg/kg,LOQ为0.5 mg/kg;各组分的平均回收率为88.10%~94.73%,相对标准偏差(relative standard deviations,RSDs)≤5.2%。结论本方法具有快速简单、高灵敏度、重现性好等优点,对糕点中抗氧化剂含量的检测具有很好的实用价值,同时为糕点的质量安全提供了有效的技术支持。Objective To establish a method for the determination of 7 kinds of natural antioxidants and 8 kinds of synthetic antioxidants in pastry by solid phase extraction high-performance liquid chromatography.Methods The targets were extracted with 1%acetonitrile acetate solution,purified with pass-through HLB solid-phase extraction column,and the purified solution was concentrated for High-performance liquid chromatography analysis.The 15kinds of targets were eluted with 0.5%formic acid in methanol as mobile phase gradient,separated by C18 column,and determined at 280 nm by diode array detector.Results There was a good linear relationship among the 15 kinds of antioxidant components in the range of 1–50μg/mL.The limit of detection(LOD)of epigallocatechin was 2 mg/kg,and the limit of quantification(LOQ)was 5 mg/kg,the LOD and LOQ of the other 14 compounds were 0.2 mg/kg and 0.5 mg/kg,and the average recoveries were 88.10%–94.73%,the relative standard deviations(RSDs)were not greater than 5.2%.Conclusion This study has the advantages of fast,simple,high sensitivity,good reproducibility,and has a good practical value for the determination of antioxidant content in pastry,and provides effective technical support for the quality safety of pastry.

关 键 词：高效液相色谱法 天然抗氧化成分 合成抗氧化剂 糕点 固相萃取 

分 类 号：O657.72[理学—分析化学] TS213.23[理学—化学]